Compartmentalization of Distinct cAMP Signaling Pathways in Mammalian Sperm
| العنوان: | Compartmentalization of Distinct cAMP Signaling Pathways in Mammalian Sperm |
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| المؤلفون: | Claudia Sánchez-Cárdenas, Jessica Escoffier, Alberto Darszon, Pablo E. Visconti, Ana M. Salicioni, Felipe Navarrete, Jesse Mager, Jochen Buck, Douglas Haddad, Dario Krapf, José L. De la Vega-Beltrán, Eva Wertheimer, Lonny R. Levin |
| المصدر: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| بيانات النشر: | Elsevier BV, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, Acrosome reaction, Biochemistry, Mice, chemistry.chemical_compound, Cyclic AMP, GTP-Binding Protein alpha Subunits, Gs, Enzyme Inhibitors, reproductive and urinary physiology, Mice, Knockout, education.field_of_study, Forskolin, Hyperactivation, heterotrimeric G proteins, purl.org/becyt/ford/3.1 [https], Bioquímica y Biología Molecular, Spermatozoa, calcium imaging, protein kinase A (PKA), Medicina Básica, purl.org/becyt/ford/3 [https], Female, Acrosome, signal transduction, Adenylyl Cyclases, Signal Transduction, endocrine system, CIENCIAS MÉDICAS Y DE LA SALUD, acrosome reaction, Biology, sperm capacitation, ADCY10, forskolin, Capacitation, cell signaling, Animals, education, Molecular Biology, Cyclic AMP-Dependent Protein Kinase Catalytic Subunits, cyclic AMP (cAMP), urogenital system, Acrosome Reaction, Adenine, Colforsin, adenilate cyclase (adenylyl cyclase), Cell Biology, Soluble adenylyl cyclase, Sperm, Cell Compartmentation, chemistry, Sperm Tail, Sperm Head, Calcium, Sperm Capacitation |
| الوصف: | Fertilization competence is acquired in the female tract in a process known as capacitation. Capacitation is needed for the activation of motility (e.g. hyperactivation) and to prepare the sperm for an exocytotic process known as acrosome reaction. While the HCO3--dependent soluble adenylyl cyclase Adcy10 plays a role in motility, less is known about the source of cAMP in the sperm head. Transmembrane adenylyl cyclases (tmACs) are another possible source of cAMP. These enzymes are regulated by stimulatory heterotrimeric Gs proteins; however, the presence of Gs or tmACs in mammalian sperm has been controversial. In this manuscript, we used Western blotting and cholera toxin-dependent ADP ribosylation to show Gs presence in the sperm head. Also, we showed that forskolin, a tmAC specific activator, induces cAMP accumulation in sperm from both WT and Adcy10 null mice. This increase is blocked by the tmAC inhibitor SQ-22536 but not by the Adcy10 inhibitor KH7. While Gs immunoreactivity and tmAC activity are detected in the sperm head, PKA is only found in the tail, where Adcy10 was previously shown to reside. Consistent with an acrosomal localization, Gs reactivity is lost in acrosome reacted sperm, and forskolin is able to increase intracellular Ca2+ and induce the acrosome reaction. Altogether, these data suggest that cAMP pathways are compartmentalized in sperm, with Gs and tmAC in the head and Adcy10 and PKA in the flagellum. Fil: Wertheimer Hermitte, Eva Victoria. University Of Massachussets; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina Fil: Krapf, Dario. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: de la Vega Beltran, José L.. Universidad Nacional Autónoma de México; México Fil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México; México Fil: Navarrete, Felipe. University Of Massachussets; Estados Unidos Fil: Haddad, Douglas. University Of Massachussets; Estados Unidos Fil: Escoffier, Jessica. University Of Massachussets; Estados Unidos Fil: Salicioni, Ana M.. University Of Massachussets; Estados Unidos Fil: Levin, Lonny R.. Cornell University; Estados Unidos Fil: Buck, Jochen. Cornell University; Estados Unidos Fil: Mager, Jesse. University Of Massachussets; Estados Unidos Fil: Darszon, Alberto. Universidad Nacional Autónoma de México; México Fil: Visconti, Pablo E.. University Of Massachussets; Estados Unidos |
| وصف الملف: | application/pdf |
| تدمد: | 0021-9258 |
| DOI: | 10.1074/jbc.m113.489476 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b37d2f18575cb8003009454cabde746c https://doi.org/10.1074/jbc.m113.489476 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b37d2f18575cb8003009454cabde746c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219258 |
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| DOI: | 10.1074/jbc.m113.489476 |