Towards a metabolomic approach to investigate iron–sulfur cluster biogenesis
| العنوان: | Towards a metabolomic approach to investigate iron–sulfur cluster biogenesis |
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| المؤلفون: | Mauro Marengo, Alex Fissore, Simonetta Oliaro‐Bosso, Salvatore Adinolfi, Annalisa Pastore |
| المصدر: | IUBMB Life. 74:715-722 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Iron-Sulfur Proteins, frataxin, Iron, CyaY, iron-sulfur cluster biogenesis, enzymatic activity, Clinical Biochemistry, Friedreich's ataxia, Cell Biology, Biochemistry, Carbon-Sulfur Lyases, desulfurase, CyaY, Friedreich's ataxia, desulfurase, enzymatic activity, frataxin, iron-sulfur cluster biogenesis, Genetics, Metabolomics, Molecular Biology, Sulfur, Protein Binding |
| الوصف: | Iron-sulfur clusters are prosthetic groups that are assembled on their acceptor proteins through a complex machine centered on a desulfurase enzyme and a transient scaffold protein. Studies to establish the mechanism of cluster formation have so far used either in vitro or in vivo methods, which have often resulted in contrasting or non-comparable results. We suggest, here, an alternative approach to study the enzymatic reaction, that is based on the combination of genetically engineered bacterial strains depleted of specific components, and the detection of the enzymatic kinetics in cellular extracts through metabolomics. Our data prove that this ex vivo approach closely reproduces the in vitro results while retaining the full complexity of the system. We demonstrate that co-presence of bacterial frataxin and iron is necessary to observe an inhibitory effect of the enzymatic activity of bacterial frataxin. Our approach provides a new powerful tool for the study of iron-sulfur cluster biogenesis. |
| تدمد: | 1521-6551 1521-6543 |
| DOI: | 10.1002/iub.2618 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b31814ee4cd1979b3a3709462c72265a https://doi.org/10.1002/iub.2618 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b31814ee4cd1979b3a3709462c72265a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15216551 15216543 |
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| DOI: | 10.1002/iub.2618 |