Simple Protocol for Generating and Genotyping Genome-Edited Mice With CRISPR-Cas9 Reagents
| العنوان: | Simple Protocol for Generating and Genotyping Genome-Edited Mice With CRISPR-Cas9 Reagents |
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| المؤلفون: | Marta Cantero, Lluis Montoliu, María Jesús del Hierro, Santiago Josa, Diego Muñoz-Santos, Matías Morín, Marcos Rubio-Fernández, Almudena Fernández, Marta Castrillo, Andrea Montero, M A Moreno-Pelayo, Julia Fernández |
| المساهمون: | Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Instituto de Salud Carlos III, Fundación Ramón Areces, Comunidad de Madrid, Centro de Investigación Biomédica en Red Cáncer (España) |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Genotyping Techniques, Computer science, Mice, Transgenic, Computational biology, Cytoplasmic injection and electroporation, In vivo gene editing, Genome, DNA sequencing, Mouse model, 03 medical and health sciences, Mice, 0302 clinical medicine, Genome editing, CRISPR, Animals, Indel, Genotyping, Gene, Next‐generation sequencing, 030304 developmental biology, Protocol (science), Gene Editing, 0303 health sciences, General Medicine, Animal models, Models, Animal, Indicators and Reagents, CRISPR-Cas Systems, 030217 neurology & neurosurgery |
| الوصف: | The simple protocol described in this article aims to provide all required information, as a comprehensive, easy‐to‐follow step‐by‐step method, to ensure the generation of the expected genome‐edited mice. Here, we provide protocols for the preparation of CRISPR‐Cas9 reagents for microinjection and electroporation into one‐cell mouse embryos to create knockout or knock‐in mouse models, and for genotyping the resulting offspring with the latest innovative next‐generation sequencing methods. LM laboratory Research Grants used for this manuscript include: MINECO (BIO2015‐70978‐R), MICINNU (RTI2018‐101223‐B‐I00), and CIBERER intramural program (ACCI:ER19P5AC756). MAMP Laboratory Research Grants used for this manuscript include: ISCIII‐FIS (PI14‐0948 and PI17‐1659); Fundación Ramón Areces (CIVP16A1849); RED RAREGENOMICS‐CM (B2017/BMD‐3721); and CIBERER intramural program (ACCI:ER19P5AC728). |
| تدمد: | 2161-2617 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2aa811279611a5e4cd7e38b0d1b5b64 https://pubmed.ncbi.nlm.nih.gov/32159922 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b2aa811279611a5e4cd7e38b0d1b5b64 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 21612617 |
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