Intravenous immunoglobulins reverse acute vaso-occlusive crises in sickle cell mice through rapid inhibition of neutrophil adhesion
| العنوان: | Intravenous immunoglobulins reverse acute vaso-occlusive crises in sickle cell mice through rapid inhibition of neutrophil adhesion |
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| المؤلفون: | Elaine Y. Chiang, Patricia A. Shi, Paul S. Frenette, Jungshan Chang |
| المصدر: | Blood. 111:915-923 |
| بيانات النشر: | American Society of Hematology, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | medicine.medical_specialty, Endothelium, Neutrophils, Red Cells, Immunology, Drug Evaluation, Preclinical, Erythrocytes, Abnormal, Mice, Transgenic, Leukocyte Rolling, Anemia, Sickle Cell, Cell Communication, Granulocyte, Biochemistry, Microcirculation, Mice, Venules, hemic and lymphatic diseases, Internal medicine, Cell Adhesion, medicine, Animals, Humans, Immunologic Factors, Vascular Diseases, Microscopy, Video, Hematology, business.industry, Immunoglobulins, Intravenous, Cell Biology, medicine.disease, Disease Models, Animal, medicine.anatomical_structure, Microscopy, Fluorescence, Bone marrow, business, Vaso-occlusive crisis, Intravital microscopy |
| الوصف: | Previous studies using intravital microscopy in a sickle cell disease (SCD) mouse model suggest that adherent leukocytes (WBCs) play a key role in vaso-occlusion by capturing circulating erythrocytes (RBCs) in venules. Commercial intravenous human gamma globulin (IVIG) given prior to the inflammatory stimuli increased microcirculatory blood flow and improved survival (Blood 2004 103(6):2397–400). To mimic the clinical situation where SCD patients seek medical attention after the onset of symptoms, we have developed an in vivo model in which the therapeutic intervention was administered after in the inflammatory challenge. Berkeley SCD bone marrow was transplanted into irradiated C57BL/6 to generate age- and gender-matched genetically identical cohorts of SCD mice. Fully engrafted male SCD mice were treated with TNF-α and prepared for intravital microscopy examination of the cremaster muscle. Seventy minutes following administration of TNF-α (0.5 μg i.p.), IVIG (800 mg/kg) or an equivalent volume of control PBS or albumin (800 mg/kg) was administered by a programmable syringe pump. Twenty minutes after IVIG, PBS, or albumin exposure, 8–10 venules were recorded over a period of 60 min with each venule recorded continuously for at least 3 min. IVIG significantly increased the number of rolling leukocytes (2–3 fold, p |
| تدمد: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2007-04-084061 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b20b5b618caf640aae6bd222b7e7b13d https://doi.org/10.1182/blood-2007-04-084061 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b20b5b618caf640aae6bd222b7e7b13d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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| DOI: | 10.1182/blood-2007-04-084061 |