Improvement of multicatalytic properties of nitrilase from Paraburkholderia graminis for efficient biosynthesis of 2‐chloronicotinic acid
| العنوان: | Improvement of multicatalytic properties of nitrilase from Paraburkholderia graminis for efficient biosynthesis of 2‐chloronicotinic acid |
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| المؤلفون: | Xiao‐Ling Tang, Yu‐Yi Li, Yue Mao, Ren‐Chao Zheng, Yu‐Guo Zheng |
| المصدر: | Biotechnology and Bioengineering. 119:3421-3431 |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Molecular Docking Simulation, Aminohydrolases, Burkholderiaceae, Nicotinic Acids, Bioengineering, Applied Microbiology and Biotechnology, Catalysis, Substrate Specificity, Biotechnology |
| الوصف: | Nitrilases are promising biocatalysts to produce high-value-added carboxylic acids through hydrolysis of nitriles. However, since the enzymes always show low activity and sometimes with poor reaction specificity toward 2-chloronicotinonitrile (2-CN), very few robust nitrilases have been reported for efficient production of 2-chloronicotinic acid (2-CA) from 2-CN. Herein, a nitrilase from Paraburkholderia graminis (PgNIT) was engineered to improve its catalytic properties. We identified the beneficial residues via computational analysis and constructed the mutant library. The positive mutants were obtained and the activity of the "best" mutant F164G/I130L/N167Y/A55S/Q260C/T133I/R199Q toward 2-CN was increased from 0.14 × 10 |
| تدمد: | 1097-0290 0006-3592 |
| DOI: | 10.1002/bit.28218 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b18059cda96ffc059e93c3728b9e6855 https://doi.org/10.1002/bit.28218 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....b18059cda96ffc059e93c3728b9e6855 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970290 00063592 |
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| DOI: | 10.1002/bit.28218 |