Methylation status of the E2 binding sites of HPV16 in cervical lesions determined with the Luminex® xMAP™ system
| العنوان: | Methylation status of the E2 binding sites of HPV16 in cervical lesions determined with the Luminex® xMAP™ system |
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| المؤلفون: | Renske D.M. Steenbergen, Peter J.F. Snijders, Chris J.L.M. Meijer, Debbie Claassen-Kramer, Denise M. Schütze, Suzanne Snellenberg |
| المساهمون: | Pathology, CCA - Oncogenesis |
| المصدر: | Snellenberg, S, Schütze, D M, Claassen-Kramer, D, Meijer, C J L M, Snijders, P J F & Steenbergen, R D M 2012, ' Methylation status of the E2 binding sites of HPV16 in cervical lesions determined with the Luminex® xMAP™ system ', Virology, vol. 422, no. 2, pp. 357-365 . https://doi.org/10.1016/j.virol.2011.11.006 Virology, 422(2), 357-365. Academic Press Inc. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Adult, Gene Expression Regulation, Viral, Uterine Cervical Neoplasms, Biology, Cervical intraepithelial neoplasia, DNA-binding protein, Methylation, Sensitivity and Specificity, Virology, E6/E7 deregulation, Transcriptional regulation, medicine, Humans, Binding site, Cells, Cultured, Aged, Cervical cancer, Human papillomavirus 16, Oncogene Proteins, Viral, DNA Methylation, Middle Aged, medicine.disease, Uterine Cervical Dysplasia, Molecular biology, Luminex xmap, E2 binding site, DNA-Binding Proteins, DNA methylation, Luminescent Measurements, Carcinoma, Squamous Cell, Female |
| الوصف: | Cervical carcinogenesis is driven by deregulated E6/E7 expression in dividing cells. A potential deregulating mechanism is methylation of E2 binding sites in the viral long control region, thereby prohibiting HPVE2-mediated transcription regulation. Here the frequency of HPV16E2BS methylation in cervical lesions (SCC, n=29; CIN3, n=17) and scrapes (controls, n=17; CIN3, n=21) was investigated. Three E2BSs were amplified using methylation independent PCR followed by specific detection of methylated CpGs using the Luminex® xMAP™ system. The frequency of E2BS1, E2BS3 and E2BS4 methylation was significantly higher in SCC compared to CIN3, i.e. 93% vs. 21% (p |
| تدمد: | 1096-0341 0042-6822 |
| DOI: | 10.1016/j.virol.2011.11.006 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1159784108edcc2a667a759fc38b7e6 https://pubmed.ncbi.nlm.nih.gov/22137333 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b1159784108edcc2a667a759fc38b7e6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10960341 00426822 |
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| DOI: | 10.1016/j.virol.2011.11.006 |