Antimicrobial activity of linear lipopeptides derived from BP100 towards plant pathogens
| العنوان: | Antimicrobial activity of linear lipopeptides derived from BP100 towards plant pathogens |
|---|---|
| المؤلفون: | Lidia Feliu, Emilio Montesinos, Laura Montesinos, Aina Baró, Marta Planas, Àngel Oliveras, Esther Badosa |
| المساهمون: | Ministerio de Ciencia e Innovación (Espanya), Ministerio de Economía y Competitividad (Espanya) |
| المصدر: | PLoS ONE, 2018, vol. 13, núm. 7, p. e0201571 Articles publicats (D-Q) DUGiDocs – Universitat de Girona instname PLoS ONE, Vol 13, Iss 7, p e0201571 (2018) PLoS ONE Recercat. Dipósit de la Recerca de Catalunya |
| بيانات النشر: | Public Library of Science (PLoS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Leaves, medicine.medical_treatment, lcsh:Medicine, Peptide, Plant Science, Pathology and Laboratory Medicine, Biochemistry, Anti-Infective Agents, Medicine and Health Sciences, lcsh:Science, chemistry.chemical_classification, Liquid Chromatography, Multidisciplinary, biology, Plant Bacterial Pathogens, Plant Anatomy, Chromatographic Techniques, Plant Fungal Pathogens, Peptides -- Synthesis, Eukaryota, Biological activity, Proteases, Plants, Antimicrobial, Bacterial Pathogens, Enzymes, Microorganismes fitopatògens, Phytopathogenic microorganisms, Medical Microbiology, Pathogens, Oligopeptides, Research Article, Xanthomonas, 030106 microbiology, Pseudomonas Syringae, Plant Pathogens, Microbial Sensitivity Tests, Research and Analysis Methods, Microbiology, Fruits, 03 medical and health sciences, Minimum inhibitory concentration, Lipopeptides, Tomatoes, Pseudomonas, Tobacco, medicine, Microbial Pathogens, Plant Diseases, Protease, Bacteria, lcsh:R, Organisms, Fungi, Fatty acid, Biology and Life Sciences, Proteins, Plant Pathology, biology.organism_classification, High Performance Liquid Chromatography, Plant Leaves, 030104 developmental biology, Cecropin, chemistry, Enzymology, Erwinia, lcsh:Q, Pèptids -- Síntesi |
| الوصف: | A collection of 36 lipopeptides were designed from the cecropin A-melittin hybrid peptide BP100 (H-Lys-Lys-Leu-Phe-Lys-Lys-Ile-Leu-Lys-Tyr-Leu-NH2) previously described with activity against phytopathogenic bacteria. These lipopeptides were synthesized on solid-phase and screened for their antimicrobial activity, toxicity and proteolytic stability. They incorporated a butanoyl, a hexanoyl or a lauroyl group at the N-terminus or at the side chain of a lysine residue placed at each position of the sequence. Their antimicrobial activity and hemolysis depended on the fatty acid length and its position. In particular, lipopeptides containing a butanoyl or a hexanoyl chain exhibited the best biological activity profile. In addition, we observed that the incorporation of the acyl group did not induce the overexpression of defense-related genes in tomato. Best lipopeptides were BP370, BP378, BP381, BP387 and BP389, which were highly active against all the pathogens tested (minimum inhibitory concentration of 0.8 to 12.5 μM), low hemolytic, low phytotoxic and significantly stable to protease degradation. This family of lipopeptides might be promising functional peptides useful for plant protection The work was supported by the following: Ministerio de Economía y Competitividad (ES); AGL2012-39880-C02-02; LM, EB, EM, LF, MP; Ministerio de Economía y Competitividad (ES); AGL2015-69876-C2-2-R; AO, LM, EB, EM, LF, MP; University of Girona (ES); MPCUDG2016/038; AO, LF, MP |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b10e8f9214ea488645746763e16ef7ec http://hdl.handle.net/10256/15880 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b10e8f9214ea488645746763e16ef7ec |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |