Colonization and plant growth promoting characterization of endophytic Pseudomonas chlororaphis strain Zong1 isolated from Sophora alopecuroides root nodules
| العنوان: | Colonization and plant growth promoting characterization of endophytic Pseudomonas chlororaphis strain Zong1 isolated from Sophora alopecuroides root nodules |
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| المؤلفون: | Ya Jun Xu, Zhan Qiang Ma, Zhen Shan Deng, Long Fei Zhao, Chang Juan Shan, Gehong Wei |
| المصدر: | Brazilian Journal of Microbiology, Vol 44, Iss 2, Pp 629-637 (2013) Brazilian Journal of Microbiology Brazilian Journal of Microbiology v.44 n.2 2013 Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Volume: 44, Issue: 2, Pages: 629-637, Published: 2013 |
| بيانات النشر: | Sociedade Brasileira de Microbiologia, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | DNA, Bacterial, Siderophore, Sophora, Root nodule, co-inoculation, PGPC, Molecular Sequence Data, Carboxylic Acids, lcsh:QR1-502, Plant Development, Siderophores, Sodium Chloride, Microbiology, Endophyte, DNA, Ribosomal, Plant Roots, lcsh:Microbiology, Phosphates, Pseudomonas, RNA, Ribosomal, 16S, Antibiosis, Endophytes, Cluster Analysis, Phylogeny, biology, Strain (chemistry), Indoleacetic Acids, Inoculation, Fungi, Temperature, Sequence Analysis, DNA, Hydrogen-Ion Concentration, biology.organism_classification, Pseudomonas chlororaphis, colonization, Anti-Bacterial Agents, the gpf-marker, endophyte, Research Paper |
| الوصف: | The endophytic strain Zong1 isolated from root nodules of the legume Sophora alopecuroides was characterized by conducting physiological and biochemical tests employing gfp-marking, observing their plant growth promoting characteristics (PGPC) and detecting plant growth parameters of inoculation assays under greenhouse conditions. Results showed that strain Zong1 had an effective growth at 28 oC after placed at 4-60 oC for 15 min, had a wide range pH tolerance of 6.0-11.0 and salt tolerance up to 5% of NaCl. Zong1 was resistant to the following antibiotics (μg/mL): Phosphonomycin (100), Penicillin (100) and Ampicillin (100). It could grow in the medium supplemented with 1.2 mmol/L Cu, 0.1% (w/v) methylene blue and 0.1-0.2% (w/v) methyl red, respectively. Zong1 is closely related to Pseudomonas chlororaphis based on analysis the sequence of 16S rRNA gene. Its expression of the gfp gene indicated that strain Zong1 may colonize in root or root nodules and verified by microscopic observation. Furthermore, co-inoculation with Zong1 and SQ1 (Mesorhizobium sp.) showed significant effects compared to single inoculation for the following PGPC parameters: siderophore production, phosphate solubilization, organic acid production, IAA production and antifungal activity in vitro. These results suggest strains P. chlororaphi Zong1 and Mesorhizobium sp. SQ1 have better synergistic or addictive effect. It was noteworthy that each growth index of co-inoculated Zong1+SQ1 in growth assays under greenhouse conditions is higher than those of single inoculation, and showed a significant difference (p < 0.05) when compared to a negative control. Therefore, as an endophyte P. chlororaphis Zong1 may play important roles as a potential plant-growth promoting agent. |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 1678-4405 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b07dbc28cca2521040be5966383bbe56 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200043&lng=en&tlng=en |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b07dbc28cca2521040be5966383bbe56 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16784405 |
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