Human pluripotent stem cell–derived erythropoietin-producing cells ameliorate renal anemia in mice
| العنوان: | Human pluripotent stem cell–derived erythropoietin-producing cells ameliorate renal anemia in mice |
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| المؤلفون: | Akira Niwa, Tatsutoshi Nakahata, Shin Ichi Mae, Azusa Hoshina, Maki Kotaka, Naoko Katagiri, Megumu K. Saito, Hirofumi Hitomi, Kenji Osafune, Akira Nishiyama, Takafumi Toyohara, Asadur Rahman, Tomoko Kasahara, Daisuke Nakano |
| المصدر: | Science Translational Medicine. 9 |
| بيانات النشر: | American Association for the Advancement of Science (AAAS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Pluripotent Stem Cells, 0301 basic medicine, medicine.medical_specialty, Anemia, Human Embryonic Stem Cells, Induced Pluripotent Stem Cells, Kidney, Mice, 03 medical and health sciences, hemic and lymphatic diseases, Internal medicine, medicine, Animals, Humans, Erythropoiesis, Insulin-Like Growth Factor I, Progenitor cell, Induced pluripotent stem cell, Erythropoietin, Cell Proliferation, business.industry, Cell Differentiation, Mouse Embryonic Stem Cells, General Medicine, medicine.disease, Cell Hypoxia, Transplantation, Disease Models, Animal, Haematopoiesis, Red blood cell, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Cancer research, business, Stem Cell Transplantation, Kidney disease, medicine.drug |
| الوصف: | The production of erythropoietin (EPO) by the kidneys, a principal hormone for the hematopoietic system, is reduced in patients with chronic kidney disease (CKD), eventually resulting in severe anemia. Although recombinant human EPO treatment improves anemia in patients with CKD, returning to full red blood cell production without fluctuations does not always occur. We established a method to generate EPO-producing cells from human induced pluripotent stem cells (hiPSCs) by modifying previously reported hepatic differentiation protocols. These cells showed increased EPO expression and secretion in response to low oxygen conditions, prolyl hydroxylase domain-containing enzyme inhibitors, and insulin-like growth factor 1. The EPO protein secreted from hiPSC-derived EPO-producing (hiPSC-EPO) cells induced the erythropoietic differentiation of human umbilical cord blood progenitor cells in vitro. Furthermore, transplantation of hiPSC-EPO cells into mice with CKD induced by adenine treatment improved renal anemia. Thus, hiPSC-EPO cells may be a useful tool for clarifying the mechanisms of EPO production and may be useful as a therapeutic strategy for treating renal anemia. |
| تدمد: | 1946-6242 1946-6234 |
| DOI: | 10.1126/scitranslmed.aaj2300 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b03b57ec3ef2b444ff61594c88706c49 https://doi.org/10.1126/scitranslmed.aaj2300 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....b03b57ec3ef2b444ff61594c88706c49 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19466242 19466234 |
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| DOI: | 10.1126/scitranslmed.aaj2300 |