High-throughput protein nanopatterning
| العنوان: | High-throughput protein nanopatterning |
|---|---|
| المؤلفون: | Elisa Riedo, Xiangyu Liu, Edoardo Albisetti, Rein V. Ulijn, Annalisa Calò, Mohit Kumar, Marcus Weck, Kylie B. Manning, Elisabeth Elacqua, Xiaouri Zheng |
| المصدر: | Faraday Discussions. 219:33-43 |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Materials science, Fabrication, Static Electricity, Thermolysin, Bacillus, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Fluorescence microscope, Physical and Theoretical Chemistry, Throughput (business), Amination, Fluorescent Dyes, Resolution (electron density), Bioprinting, Enzymes, Immobilized, 021001 nanoscience & nanotechnology, Chip, Fluorescence, 0104 chemical sciences, Methacrylates, 0210 nano-technology, Scanning probe lithography |
| الوصف: | High-throughput and large-scale patterning of enzymes with sub-10 nm resolution, the size range of individual protein molecules, is crucial for propelling advancement in a variety of areas, from the development of chip-based biomolecular nano-devices to molecular-level studies of cell biology. Despite recent developments in bio-nanofabrication technology, combining 10 nm resolution with high-throughput and large-scale patterning of enzymes is still an open challenge. Here, we demonstrate a high resolution and high-throughput patterning method to generate enzyme nanopatterns with sub-10 nm resolution by using thermochemical scanning probe lithography (tc-SPL). First, tc-SPL is used to generate amine patterns on a methacrylate copolymer film. Thermolysin enzymes functionalized with sulfonate-containing fluorescent labels (Alexa-488) are then directly immobilized onto the amine patterns through electrostatic interaction. Enzyme patterns with sub-10 nm line width are obtained as evidenced by atomic force microscopy (AFM) and fluorescence microscopy. Moreover, we demonstrate large-scale and high throughput (0.13 × 0.1 mm2 at a throughput of 5.2 × 104 μm2 h-1) patterning of enzymes incorporating 10 nm detailed pattern features. This straightforward and high-throughput method of fabricating enzyme nanopatterns will have a significant impact on future bio-nanotechnology applications and molecular-level biological studies. By scaling up using parallel probes, tc-SPL is promising for implementation to scale up the fabrication of nano-biodevices. |
| تدمد: | 1364-5498 1359-6640 |
| DOI: | 10.1039/c9fd00025a |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af5f64e693df4055fb2a5ca5126d04a9 https://doi.org/10.1039/c9fd00025a |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....af5f64e693df4055fb2a5ca5126d04a9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13645498 13596640 |
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| DOI: | 10.1039/c9fd00025a |