The NAD Kinase Slr0400 Functions as a Growth Repressor in Synechocystis sp. PCC 6803
| العنوان: | The NAD Kinase Slr0400 Functions as a Growth Repressor in Synechocystis sp. PCC 6803 |
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| المؤلفون: | Yasuko Kaneko, Yuuma Ishikawa, Cédric Cassan, Yves Gibon, Yoshitaka Nishiyama, Yukako Hihara, Maki Kawai-Yamada, Masatoshi Yamaguchi, Koki Yuasa, Aikeranmu Kadeer, Nozomu Sato, Toshiki Ishikawa, Kintake Sonoike, Atsuko Miyagi, Hiroko Takahashi |
| المساهمون: | Heinrich Heine Universität Düsseldorf = Heinrich Heine University [Düsseldorf], Institute of Transformative Bio-Molecules [Nagoya, Japan], Nagoya University, Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan, Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of Science and Engineering [University of Waseda], Waseda University, The KAKENHI (Grant Numbers 17H05714, 26292190, T18H02165 and 19H04715 to M.K.-Y., 16H06552 and 16H06553 to K.S., and 18J11305 to Y.I.) |
| المصدر: | Plant and Cell Physiology Plant and Cell Physiology, Oxford University Press (OUP), 2021, 62 (4), pp.668-677. ⟨10.1093/pcp/pcab023⟩ |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, Light, Physiology, [SDV]Life Sciences [q-bio], Mutant, Arabidopsis, Repressor, Plant Science, Nicotinamide adenine dinucleotide, Cyanobacteria, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Adenosine Triphosphate, Bacterial Proteins, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Glycolysis, Photosynthesis, Growth repressor, NAD kinase, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, biology, Arabidopsis Proteins, Synechocystis, Genetic Complementation Test, Cell Biology, General Medicine, Slr0400, biology.organism_classification, Plants, Genetically Modified, Citric acid cycle, Adenosine Diphosphate, Phosphotransferases (Alcohol Group Acceptor), Enzyme, Phenotype, chemistry, Biochemistry, Synechocysti ssp. PCC 6803, Mutation, NAD+ kinase, 010606 plant biology & botany |
| الوصف: | NADP+, the phosphorylated form of nicotinamide adenine dinucleotide (NAD), plays an essential role in many cellular processes. NAD kinase (NADK), which is conserved in all living organisms, catalyzes the phosphorylation of NAD+ to NADP+. However, the physiological role of phosphorylation of NAD+ to NADP+ in the cyanobacterium Synechocystis remains unclear. In this study, we report that slr0400, an NADK-encoding gene in Synechocystis, functions as a growth repressor under light-activated heterotrophic growth conditions and light and dark cycle conditions in the presence of glucose. We show, via characterization of NAD(P)(H) content and enzyme activity, that NAD+ accumulation in slr0400-deficient mutant results in the unsuppressed activity of glycolysis and tricarboxylic acid (TCA) cycle enzymes. In determining whether Slr0400 functions as a typical NADK, we found that constitutive expression of slr0400 in an Arabidopsis nadk2-mutant background complements the pale-green phenotype. Moreover, to determine the physiological background behind the growth advantage of mutants lacking slr04000, we investigated the photobleaching phenotype of slr0400-deficient mutant under high-light conditions. Photosynthetic analysis found in the slr0400-deficient mutant resulted from malfunctions in the Photosystem II (PSII) photosynthetic machinery. Overall, our results suggest that NADP(H)/NAD(H) maintenance by slr0400 plays a significant role in modulating glycolysis and the TCA cycle to repress the growth rate and maintain the photosynthetic capacity. |
| تدمد: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcab023⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af5a127a860369afd9cb58b7fb3952f5 https://pubmed.ncbi.nlm.nih.gov/33560438 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....af5a127a860369afd9cb58b7fb3952f5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14719053 00320781 |
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| DOI: | 10.1093/pcp/pcab023⟩ |