Effects of a GSK-3β inhibitor on the renal expression levels of RANK, RANKL and NF-κB in a rat model of diabetic nephropathy
| العنوان: | Effects of a GSK-3β inhibitor on the renal expression levels of RANK, RANKL and NF-κB in a rat model of diabetic nephropathy |
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| المؤلفون: | Huan Yao, Lu Meng, Yang Zhang, Li‑Xin Shi, Yan‑Ni Yu, Yi‑Guo Long, Hua Yang, Long Li, Yi‑Xia Zhou, Li‑Sa Lv |
| المصدر: | Experimental and Therapeutic Medicine. 11:2495-2502 |
| بيانات النشر: | Spandidos Publications, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, medicine.medical_specialty, 030209 endocrinology & metabolism, Diabetic nephropathy, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Immunology and Microbiology (miscellaneous), Internal medicine, medicine, Receptor, Kidney, Oncogene, biology, RANK Ligand, NF-κB, Articles, General Medicine, medicine.disease, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, chemistry, RANKL, biology.protein, Immunohistochemistry |
| الوصف: | The present study aimed to investigate the effects of glycogen synthase kinase-3β (GSK-3β) on the expression levels of receptor activator of nuclear factor (NF)-κB (RANK), RANK ligand (RANKL) and NF-κB in the renal tissues of rats modeling diabetic nephropathy (DN). The rats were allocated at random into three groups, as follows: Normal control group (NC), the DN model group (DNM group) and the DN model lithium chloride (LiCl) intervention group (DNI group). Urinary proteins were examined by staining with the Coomassie Brilliant Blue dye for 24 h. Histochemical analyses of kidney tissue sections were conducted using hematoxylin and eosin staining, after which the kidney pathology of the rats was observed. In addition, the mRNA and protein expression levels of GSK-3β, RANK, RANKL and NF-κB in the renal tissues were detected using reverse transcription-quantitative polymerase chain reaction and immunohistochemistry, respectively. As compared with the NC group, the level of urinary protein was significantly increased in the DNM group (P |
| تدمد: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2016.3184 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af1c57bea27ca70817491911e1394450 https://doi.org/10.3892/etm.2016.3184 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....af1c57bea27ca70817491911e1394450 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921015 17920981 |
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| DOI: | 10.3892/etm.2016.3184 |