التفاصيل البيبلوغرافية
| العنوان: |
Vesicular Glutamate Transporter Expression Level Affects Synaptic Vesicle Release Probability at Hippocampal Synapses in Culture |
| المؤلفون: |
Frauke Ackermann, Melissa A. Herman, Thorsten Trimbuch, Christian Rosenmund |
| المصدر: |
Journal of Neuroscience
The journal of neuroscience 34(35), 11781-11791 (2014). doi:10.1523/JNEUROSCI.1444-14.2014 |
| سنة النشر: |
2014 |
| مصطلحات موضوعية: |
Patch-Clamp Techniques, metabolism [Hippocampus], Neurotransmission, Biology, Hippocampal formation, Synaptic vesicle, Hippocampus, Synaptic Transmission, 03 medical and health sciences, Mice, 0302 clinical medicine, Microscopy, Electron, Transmission, Animals, ddc:610, metabolism [Synaptic Vesicles], Cells, Cultured, 030304 developmental biology, Mice, Knockout, 0303 health sciences, General Neuroscience, Vesicle, Glutamate receptor, Transporter, metabolism [Synapses], Articles, Immunohistochemistry, Cell biology, Vesicular transport protein, Mice, Inbred C57BL, Electrophysiology, physiology [Synaptic Transmission], biosynthesis [Vesicular Glutamate Transport Protein 1], Synapses, Vesicular Glutamate Transport Protein 1, Synaptic Vesicles, Neuroscience, 030217 neurology & neurosurgery |
| الوصف: |
The vesicular glutamate transporter (VGLUT) plays an essential role in synaptic transmission by filling vesicles with glutamate. At mammalian synapses, VGLUT expression level determines the amount of glutamate packaged into vesicles, and the specific paralog of VGLUT expressed affects the release probability. In this study, we investigate whether there is a link between the number of VGLUTs on vesicles and release probability. We used a combination of electrophysiology and imaging techniques in cultured mouse hippocampal neurons where the VGLUT expression level has been severely altered. We found that vesicles with drastically reduced VGLUT expression were released with a lower probability. This deficit in release could only be rescued by a functional transporter, suggesting that the transport function, and not the molecular interactions, of the protein affects vesicle release. Based on these data, we propose a novel means of presynaptic vesicle release regulation—the intravesicular glutamate fill state of the vesicle. |
| اللغة: |
English |
| تدمد: |
0270-6474 |
| DOI: |
10.1523/JNEUROSCI.1444-14.2014 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af0d2d8426ed00aa57abbdfb40a30414 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....af0d2d8426ed00aa57abbdfb40a30414 |
| قاعدة البيانات: |
OpenAIRE |