Dual Stimulation of Ras/Mitogen-Activated Protein Kinase and Rhoa by Cell Adhesion to Fibronectin Supports Growth Factor–Stimulated Cell Cycle Progression
| العنوان: | Dual Stimulation of Ras/Mitogen-Activated Protein Kinase and Rhoa by Cell Adhesion to Fibronectin Supports Growth Factor–Stimulated Cell Cycle Progression |
|---|---|
| المؤلفون: | Kenneth M. Yamada, Arnoud Sonnenberg, Erik H.J. Danen, Petra Sonneveld |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | Rockefeller University Press, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | MAPK/ERK pathway, RHOA, medicine.medical_treatment, Cell Cycle Proteins, Retinoblastoma Protein, Culture Media, Serum-Free, S Phase, Mice, Cyclin D1, Growth Substances, Cytoskeleton, biology, Cell Cycle, Retinoblastoma protein, 3T3 Cells, Cell cycle, Cell biology, DNA-Binding Proteins, ras GTPase-Activating Proteins, Original Article, Mitogen-Activated Protein Kinases, Signal transduction, Transcription Factor DP1, Signal Transduction, Cyclin-Dependent Kinase Inhibitor p21, integrin, Transfection, Models, Biological, fibronectin, Rho, Cyclins, Cell Adhesion, medicine, Animals, RNA, Messenger, Protein kinase A, Growth factor, G1 Phase, Cell Biology, G1 cell cycle, Actins, E2F Transcription Factors, Fibronectins, Enzyme Activation, Fibronectin, biology.protein, Cancer research, Carrier Proteins, rhoA GTP-Binding Protein, Retinoblastoma-Binding Protein 1, Transcription Factors |
| الوصف: | In cellular transformation, activated forms of the small GTPases Ras and RhoA can cooperate to drive cells through the G1 phase of the cell cycle. Here, we show that a similar but substrate-regulated mechanism is involved in the anchorage-dependent proliferation of untransformed NIH-3T3 cells. Among several extracellular matrix components tested, only fibronectin supported growth factor–induced, E2F-dependent S phase entry. Although all substrates supported the mitogen-activated protein kinase (MAPK) response to growth factors, RhoA activity was specifically enhanced on fibronectin. Moreover, induction of cyclin D1 and suppression of p21Cip/Waf occurred specifically, in a Rho-dependent fashion, in cells attached to fibronectin. This ability of fibronectin to stimulate both Ras/MAPK- and RhoA-dependent signaling can explain its potent cooperation with growth factors in the stimulation of cell cycle progression. |
| تدمد: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.151.7.1413 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af01f95876fc0b8ab6e52ac94e99765e https://doi.org/10.1083/jcb.151.7.1413 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....af01f95876fc0b8ab6e52ac94e99765e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
|---|---|
| DOI: | 10.1083/jcb.151.7.1413 |