Morphological and enzymatic study of membrane-bound vesicles from the lumen of the rat epididymis
| العنوان: | Morphological and enzymatic study of membrane-bound vesicles from the lumen of the rat epididymis |
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| المؤلفون: | A. Barbieri, J. C. Cavicchia, Miguel W. Fornés |
| المصدر: | Andrologia. 27:1-5 |
| بيانات النشر: | Hindawi Limited, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Male, Urology, Lumen (anatomy), Columnar Cell, Biology, Endocrinology, alpha-Mannosidase, Acetylglucosaminidase, Mannosidases, medicine, Animals, Freeze Fracturing, Arylsulfatases, Glucuronidase, Epididymis, chemistry.chemical_classification, Vesicle, Cell Membrane, General Medicine, Anatomy, beta-Galactosidase, Epithelium, Rats, Microscopy, Electron, medicine.anatomical_structure, Enzyme, chemistry, Transmission electron microscopy, Ultrastructure, Biophysics |
| الوصف: | The epithelium of caput and cauda epididymidis of the rat was studied with transmission electron microscopy (TEM) and freeze-fracture techniques. In thin sections of both zones, the tissue consisted mainly of tall columnar cells (principal cells) with long sterocilia. Clusters of small membrane-bound vesicles were located in the lumen between or immediately over the stereocilia. Freeze-fracture replicas also displayed groups of smooth-surface vesicles in the same location. Membrane-bound vesicles isolated from the lumen of the rat epididymis were studied by TEM. In thin sections, some of them contained an electron dense material and others looked empty. In addition, the hydrolases: beta-galactosidase, N-acetyl-glycosaminidase, alpha-mannosidase, aryl-sulfatase and beta-glucuronidase were detectable in pellets of vesicles treated with Triton X-100. The results presented here indicate the presence of membrane-bound vesicles observed by two different methodologies in the rat epididymal fluid and demonstrate five glycosidases in their content. |
| تدمد: | 1439-0272 0303-4569 |
| DOI: | 10.1111/j.1439-0272.1995.tb02087.x |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae608f0be77aca123a09a5654235ede2 https://doi.org/10.1111/j.1439-0272.1995.tb02087.x |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....ae608f0be77aca123a09a5654235ede2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14390272 03034569 |
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| DOI: | 10.1111/j.1439-0272.1995.tb02087.x |