The Combination of N-Acetyl Cysteine, Alpha-Lipoic Acid, and Bromelain Shows High Anti-Inflammatory Properties in NovelIn VivoandIn VitroModels of Endometriosis
| العنوان: | The Combination of N-Acetyl Cysteine, Alpha-Lipoic Acid, and Bromelain Shows High Anti-Inflammatory Properties in NovelIn VivoandIn VitroModels of Endometriosis |
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| المؤلفون: | Roberta Bulla, Chiara Agostinis, Giorgio Zauli, Sonia Zorzet, R. De Leo, F. De Seta |
| المساهمون: | Agostinis, C., Zorzet, Sonia, De Leo, R., Zauli, G., DE SETA, Francesco, Bulla, Roberta |
| المصدر: | Mediators of Inflammation, Vol 2015 (2015) Mediators of Inflammation |
| بيانات النشر: | Hindawi Limited, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Pathology, Anti-Inflammatory Agents, Apoptosis, Mice, SCID, Pharmacology, Acetylcysteine, Mice, and bromelain, Endometriosi, Cells, Cultured, Caspase, Microscopy, Cultured, Thioctic Acid, biology, Bromelains, endothelial cells, N-acetyl cystein, endothelial cell, Female, Tumor necrosis factor alpha, Research Article, lcsh:RB1-214, medicine.drug, medicine.medical_specialty, Article Subject, medicine.drug_class, Cells, Immunology, Endometriosis, Vascular Cell Adhesion Molecule-1, SCID, Fluorescence, Anti-inflammatory, NO, Proinflammatory cytokine, In vivo, lcsh:Pathology, medicine, Animals, Humans, Animal, Tumor Necrosis Factor-alpha, Cell Biology, inflammation, alpha-lipoic acid, and bromelain, business.industry, alpha-lipoic acid, Uterus, Disease Models, Animal, Endothelial Cells, Inflammation, Microscopy, Fluorescence, In vitro, Disease Models, biology.protein, business |
| الوصف: | To evaluate the efficacy of an association of N-acetyl cystein, alpha-lipoic acid, and bromelain (NAC/LA/Br) in the treatment of endometriosis we set up a newin vivomurine model. We explored the anti-inflammatory and proapoptotic effect of this combination on human endometriotic endothelial cells (EECs) and on endothelial cells isolated from normal uterus (UtMECs). We implanted fragments of human endometriotic cysts intraperitoneally into SCID mice to evaluate the efficacy of NAC/LA/Br treatment. UtMECs and EECs, untreated or treated with NAC/LA/Br, were activated with the proinflammatory stimulus TNF-αand their response in terms of VCAM1 expression was evaluated. The proapoptotic effect of higher doses of NAC/LA/Br on UtMECs and EECs was measured with a fluorogenic substrate for activated caspases 3 and 7. The preincubation of EECs with NAC/LA/Br prior to cell stimulation with TNF-αprevents the upregulation of the expression of the inflammatory “marker” VCAM1. Furthermore NAC/LA/Br were able to induce EEC, but not UtMEC, apoptosis. Finally, the novel mouse model allowed us to demonstrate that mice treated with NAC/LA/Br presented a lower number of cysts, smaller in size, compared to untreated mice. Our findings suggest that these dietary supplements may have potential therapeutic uses in the treatment of chronic inflammatory diseases like endometriosis. |
| وصف الملف: | ELETTRONICO; text/xhtml |
| تدمد: | 1466-1861 0962-9351 |
| DOI: | 10.1155/2015/918089 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::adb1dd5fb0c0eeaf75ea919afd523e03 https://doi.org/10.1155/2015/918089 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....adb1dd5fb0c0eeaf75ea919afd523e03 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14661861 09629351 |
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| DOI: | 10.1155/2015/918089 |