Protein tyrosine phosphorylation and regulation of the receptor for platelet-activating factor in rat Kupffer cells. Effect of sodium vanadate
| العنوان: | Protein tyrosine phosphorylation and regulation of the receptor for platelet-activating factor in rat Kupffer cells. Effect of sodium vanadate |
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| المؤلفون: | Donald J. Hanahan, Heling Liu, Merle S. Olson, Wei Chao |
| المصدر: | Biochemical Journal. 288:777-784 |
| بيانات النشر: | Portland Press Ltd., 1992. |
| سنة النشر: | 1992 |
| مصطلحات موضوعية: | Time Factors, Kupffer Cells, Receptors, Cell Surface, Platelet Membrane Glycoproteins, Biology, Sensitivity and Specificity, Biochemistry, Dinoprostone, Receptors, G-Protein-Coupled, chemistry.chemical_compound, Cell surface receptor, Animals, Vanadate, Phosphorylation, Platelet Activating Factor, Tyrosine, Receptor, Molecular Biology, Platelet-activating factor, Temperature, Proteins, Tyrosine phosphorylation, Cell Biology, Protein-Tyrosine Kinases, Genistein, Isoflavones, Stimulation, Chemical, Rats, Kinetics, chemistry, Protein Tyrosine Phosphatases, Vanadates, Signal transduction, Research Article |
| الوصف: | Platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine, AGEPC) and sodium vanadate (a phosphotyrosine phosphatase inhibitor) induced a time- and concentration-dependent increase in phosphotyrosine in several proteins and stimulated prostaglandin (PG) E2 production in cultured rat Kupffer cells. In addition, vanadate induced a decrease in the surface expression of AGEPC receptors and, as a consequence, inhibited AGEPC-stimulated PGE2 production. The vanadate-induced decrease in the surface expression of AGEPC receptors was time- and concentration-dependent and was partially prevented by genistein, a putative tyrosine kinase inhibitor. Upon removal of vanadate from the culture medium and re-incubation of cells in vanadate-free medium, the surface AGEPC receptors were restored within 7 h after the removal of vanadate. Both AGEPC- and vanadate-stimulated PGE2 formation was attenuated by genistein. Thus the present investigation demonstrates that both AGEPC and sodium vanadate stimulate tyrosine phosphorylation of cellular proteins, and vanadate induces a decrease in the number of the surface AGEPC receptors. These results suggest that protein tyrosine phosphorylation may play a role, directly or indirectly, in the regulation of surface expression of AGEPC receptors as well as in PGE2 production in response to vanadate and AGEPC. |
| تدمد: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj2880777 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad118d9d642b1b7329463b92a5b2f0b1 https://doi.org/10.1042/bj2880777 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ad118d9d642b1b7329463b92a5b2f0b1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14708728 02646021 |
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| DOI: | 10.1042/bj2880777 |