Thirup, S S, Van, L B, Nielsen, T K & Knudsen, C R 2015, ' Structural outline of the detailed mechanism for elongation factor Ts-mediated guanine nucleotide exchange on elongation factor Tu ', Journal of Structural Biology, vol. 191, no. 1, pp. 10-21 . https://doi.org/10.1016/j.jsb.2015.06.011
Translation elongation factor EF-Tu belongs to the superfamily of guanine-nucleotide binding proteins, which play key cellular roles as regulatory switches. All G-proteins require activation via exchange of GDP for GTP to carry out their respective tasks. Often, guanine-nucleotide exchange factors are essential to this process. During translation, EF-Tu:GTP transports aminoacylated tRNA to the ribosome. GTP is hydrolyzed during this process, and subsequent reactivation of EF-Tu is catalyzed by EF-Ts. The reaction path of guanine-nucleotide exchange is structurally poorly defined for EF-Tu and EF-Ts. We have determined the crystal structures of the following reaction intermediates: two structures of EF-Tu:GDP:EF-Ts (2.2 and 1.8 A resolution), EF-Tu:PO4:EF-Ts (1.9 A resolution), EF-Tu:GDPNP:EF-Ts (2.2 A resolution) and EF-Tu:GDPNP:pulvomycin:Mg2+:EF-Ts (3.5 A resolution). These structures provide snapshots throughout the entire exchange reaction and suggest a mechanism for the release of EF-Tu in its GTP conformation. An inferred sequence of events during the exchange reaction is presented.