KIF2beta , a new kinesin superfamily protein in non-neuronal cells, is associated with lysosomes and may be implicated in their centrifugal translocation
| العنوان: | KIF2beta , a new kinesin superfamily protein in non-neuronal cells, is associated with lysosomes and may be implicated in their centrifugal translocation |
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| المؤلفون: | Santama, Niovi, Krijnse-Ĺocker, J., Griffiths, G., Noda, Y., Hirokawa, N., Dotti, C. G. |
| المساهمون: | Santama, Niovi [0000-0002-8370-8674] |
| المصدر: | EMBO Journal EMBO J. |
| بيانات النشر: | Wiley, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Intracellular Fluid, nervous system development, hippocampus, Cellular differentiation, complementary DNA, Kinesins, animal cell, Motor proteins, alternative rna splicing, kinesin, Microtubules, Hippocampus, fibroblast, Mice, newborn, animal, genetics, membrane protein, rat, Cells, Cultured, Neurons, General Neuroscience, article, Gene Expression Regulation, Developmental, Cell Differentiation, gene expression regulation, 3T3 Cells, Lamp-1,2, Immunohistochemistry, Kif2b protein, mouse, Cell biology, Isoenzymes, medicine.anatomical_structure, Endocytic vesicle, priority journal, immunohistochemistry, lysosome, protein transport, Kinesin, nerve cell, isoenzyme, Research Article, DNA, Complementary, messenger rna, Endosome, enzymology, Molecular Sequence Data, DNA sequence, embryo, Biology, General Biochemistry, Genetics and Molecular Biology, intracellular fluid, detergent, Microtubule, Lysosome, medicine, Animals, controlled study, cell strain 3T3, Amino Acid Sequence, immunofluorescence, protein expression, endosome, Molecular Biology, mouse, centrifugation, Cell Nucleus, cell culture, nonhuman, electron microscopy, Base Sequence, General Immunology and Microbiology, isolation and purification, cell nucleus, nucleotide sequence, Sequence Analysis, DNA, Fibroblasts, protein family, amino acid sequence, cell differentiation, Membrane protein, molecular genetics, physiology, Axoplasmic transport, pathology, genetic transfection, biosynthesis, Lysosomes, metabolism |
| الوصف: | Lysosomes concentrate juxtanuclearly in the region around the microtubule-organizing center by interaction with microtubules. Different experimental and physiological conditions can induce these organelles to move to the cell periphery by a mechanism implying a plus-end-directed microtubule-motor protein (a kinesin-like motor). The responsible kinesin-superfamily protein, however, is unknown. We have identified a new mouse isoform of the kinesin superfamily, KIF2β, an alternatively spliced isoform of the known, neuronal kinesin, KIF2. Developmental expression pattern and cell-type analysis in vivo and in vitro reveal that KIF2β is abundant at early developmental stages of the hippocampus but is then downregulated in differentiated neuronal cells, and it is mainly or uniquely expressed in non-neuronal cells while KIF2 remains exclusively neuronal. Electron microscopy of mouse fibroblasts and immunofluorescence of KIF2β-transiently-transfected fibroblasts show KIF2 and KIF2β primarily associated with lysosomes, and this association can be disrupted by detergent treatment. In KIF2β-overexpressing cells, lysosomes (labeled with anti-lysosome-associated membrane protein-1) become abnormally large and peripherally located at some distance from their usual perinuclear positions. Overexpression of KIF2 or KIF2β does not change the size or distribution of early, late and recycling endosomes nor does overexpression of different kinesin superfamily proteins result in changes in lysosome size or positioning. These results implicate KIF2β as a motor responsible for the peripheral translocation of lysosomes. 17 5855 5867 Cited By :50 |
| تدمد: | 1460-2075 |
| DOI: | 10.1093/emboj/17.20.5855 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aca69135a1f7e0e856d855864917c3cc https://doi.org/10.1093/emboj/17.20.5855 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....aca69135a1f7e0e856d855864917c3cc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602075 |
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| DOI: | 10.1093/emboj/17.20.5855 |