Plumbagin-Loaded Nanoemulsion Drug Delivery Formulation and Evaluation of Antiproliferative Effect on Prostate Cancer Cells
| العنوان: | Plumbagin-Loaded Nanoemulsion Drug Delivery Formulation and Evaluation of Antiproliferative Effect on Prostate Cancer Cells |
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| المؤلفون: | John Welsh, Adrian Chrastina, Gaelle Rondeau, Véronique Baron, Per Borgström, Parisa Abedinpour |
| المصدر: | BioMed Research International BioMed Research International, Vol 2018 (2018) |
| بيانات النشر: | Hindawi, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Article Subject, lcsh:Medicine, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, chemistry.chemical_compound, Mice, Surface-Active Agents, 0302 clinical medicine, Drug Delivery Systems, Pulmonary surfactant, Dynamic light scattering, Cell Line, Tumor, Distribution (pharmacology), Animals, Solubility, Particle Size, Cell Proliferation, Chromatography, General Immunology and Microbiology, Cell Death, lcsh:R, Prostatic Neoplasms, General Medicine, Plumbagin, Oleic acid, Drug Liberation, 030104 developmental biology, chemistry, 030220 oncology & carcinogenesis, Drug delivery, Nanoparticles, Emulsions, Particle size, Research Article, Naphthoquinones |
| الوصف: | Background. Plumbagin, a medicinal plant-derived 5-hydroxy-2-methyl-1,4-naphthoquinone, is an emerging drug with a variety of pharmacological effects, including potent anticancer activity. We have previously shown that plumbagin improves the efficacy of androgen deprivation therapy (ADT) in prostate cancer and it is now being evaluated in phase I clinical trial. However, the development of formulation of plumbagin as a compound with sparing solubility in water is challenging. Methods. We have formulated plumbagin-loaded nanoemulsion using pneumatically controlled high pressure homogenization of oleic acid dispersions with polyoxyethylene (20) sorbitan monooleate as surfactant. Nanoemulsion formulations were characterized for particle size distribution by dynamic light scattering (DLS). The kinetics of in vitro drug release was determined by equilibrium dialysis. Anticancer activity toward prostate cancer cells PTEN-P2 was assessed by MTS (Owen’s reagent) assay. Results. Particle size distribution of nanoemulsions is tunable and depends on the surfactant concentration. Nanoemulsion formulations of plumbagin with 1-3.5% (w/w) of surfactant showed robust stability of size distribution over time. Plumbagin-loaded nanoemulsion with average hydrodynamic diameter of 135 nm showed exponential release of plumbagin with a half-life of 6.1 h in simulated gastric fluid, 7.0 h in simulated intestinal fluid, and displayed enhanced antiproliferative effect toward prostate cancer cells PTEN-P2 compared to free plumbagin. Conclusion. High drug-loading capacity, retention of nanoparticle size, kinetics of release under simulated physiological conditions, and increased antiproliferative activity indicate that oleic-acid based nanoemulsion formulation is a suitable delivery system of plumbagin. |
| وصف الملف: | text/xhtml |
| اللغة: | English |
| تدمد: | 2314-6141 2314-6133 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab9911d478634851f4cf52f7afff04e9 http://europepmc.org/articles/PMC6252225 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ab9911d478634851f4cf52f7afff04e9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146141 23146133 |
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