Impact of extrinsic incubation temperature on natural selection during Zika virus infection of Aedes aegypti and Aedes albopictus
| العنوان: | Impact of extrinsic incubation temperature on natural selection during Zika virus infection of Aedes aegypti and Aedes albopictus |
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| المؤلفون: | Gregory D. Ebel, Michael C. Young, Gareth Halladay, Deedra J Murrieta, Joseph R. Fauver, James Weger-Lucarelli, Selene M. Garcia-Luna, Reyes A. Murrieta, Claudia Rückert, Alex Gendernalik |
| المصدر: | PLoS Pathogens, Vol 17, Iss 11, p e1009433 (2021) PLoS Pathogens |
| بيانات النشر: | Public Library of Science (PLoS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Viral Diseases, Physiology, Disease Vectors, Mosquitoes, Zika virus, Body Temperature, Medical Conditions, Aedes, Chlorocebus aethiops, Medicine and Health Sciences, Biology (General), Incubation, education.field_of_study, biology, Zika Virus Infection, Temperature, Eukaryota, Viral Load, Body Fluids, Insects, Infectious Diseases, Physiological Parameters, Arboviral Infections, Viruses, Anatomy, Research Article, Aedes albopictus, Arthropoda, QH301-705.5, Immunology, Population, Zoology, Aedes aegypti, Mosquito Vectors, Aedes Aegypti, Arbovirus, Microbiology, Virology, medicine, Genetics, Animals, Selection, Genetic, education, Saliva, Molecular Biology, Vero Cells, Evolutionary Biology, Population Biology, fungi, Organisms, Biology and Life Sciences, Zika Virus, RC581-607, biology.organism_classification, medicine.disease, Invertebrates, Viral Replication, Insect Vectors, Species Interactions, Vector (epidemiology), Parasitology, Genetic Fitness, Immunologic diseases. Allergy, Entomology, Arboviruses, Population Genetics |
| الوصف: | Arthropod-borne viruses (arboviruses) require replication across a wide range of temperatures to perpetuate. While vertebrate hosts tend to maintain temperatures of approximately 37°C—40°C, arthropods are subject to ambient temperatures which can have a daily fluctuation of > 10°C. Temperatures impact vector competence, extrinsic incubation period, and mosquito survival unimodally, with optimal conditions occurring at some intermediate temperature. In addition, the mean and range of daily temperature fluctuations influence arbovirus perpetuation and vector competence. The impact of temperature on arbovirus genetic diversity during systemic mosquito infection, however, is poorly understood. Therefore, we determined how constant extrinsic incubation temperatures of 25°C, 28°C, 32°C, and 35°C control Zika virus (ZIKV) vector competence and population dynamics within Aedes aegypti and Aedes albopictus mosquitoes. We also examined fluctuating temperatures which better mimic field conditions in the tropics. We found that vector competence varied in a unimodal manner for constant temperatures peaking between 28°C and 32°C for both Aedes species. Transmission peaked at 10 days post-infection for Aedes aegypti and 14 days for Aedes albopictus. Conversely, fluctuating temperature decreased vector competence. Using RNA-seq to characterize ZIKV population structure, we identified that temperature alters the selective environment in unexpected ways. During mosquito infection, constant temperatures more often elicited positive selection whereas fluctuating temperatures led to strong purifying selection in both Aedes species. These findings demonstrate that temperature has multiple impacts on ZIKV biology, including major effects on the selective environment within mosquitoes. Author summary Arthropod-borne viruses (arboviruses) have emerged in recent decades due to complex factors that include increases in international travel and trade, the breakdown of public health infrastructure, land use changes, and many others. Climate change also has the potential to shift the geographical ranges of arthropod vectors, consequently increasing the global risk of arbovirus infection. Changing temperatures may alter the virus-host interaction, ultimately resulting in the emergence of new viruses and virus genotypes in new areas. Therefore, we sought to characterize how temperature (both constant and fluctuating) alters the ability of Aedes aegypti and Aedes albopictus to transmit Zika virus, and how it influences virus populations within mosquitoes. We found that intermediate temperatures maximize virus transmission compared to more extreme and fluctuating temperatures. Constant temperatures increased positive selection on virus genomes, while fluctuating temperatures strengthened purifying selection. Our studies provide evidence that in addition to altering vector competence, temperature significantly influences natural selection within mosquitoes. |
| اللغة: | English |
| تدمد: | 1553-7374 1553-7366 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa16a9ec5e6191598aa2168752fe9955 https://doaj.org/article/7a9bf5d988624cbe86e0049f4691cd68 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....aa16a9ec5e6191598aa2168752fe9955 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15537374 15537366 |
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