Matrix compliance and the regulation of cytokinesis
| العنوان: | Matrix compliance and the regulation of cytokinesis |
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| المؤلفون: | Scott Varney, Savitha Sambandamoorthy, Jonathan M. Zuidema, Susan E. LaFlamme, Shomita S. Mathew-Steiner, Livingston Van De Water, Ryan J. Gilbert |
| المصدر: | Biology Open, Vol 4, Iss 7, Pp 885-892 (2015) Biology Open |
| بيانات النشر: | The Company of Biologists, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Integrins, Cell division, Cell growth, QH301-705.5, Science, Integrin, Biology, Matrix (biology), Bioinformatics, Cell junction, General Biochemistry, Genetics and Molecular Biology, Cell biology, Matrix compliance, biology.protein, Biology (General), General Agricultural and Biological Sciences, Cell adhesion, Cytokinesis, Tissue homeostasis, Research Article |
| الوصف: | Integrin-mediated cell adhesion to the ECM regulates many physiological processes in part by controlling cell proliferation. It is well established that many normal cells require integrin-mediated adhesion to enter S phase of the cell cycle. Recent evidence indicates that integrins also regulate cytokinesis. Mechanical properties of the ECM can dictate entry into S phase; however, it is not known whether they also can affect the successful completion of cell division. To address this issue, we modulated substrate compliance using fibronectin-coated acrylamide-based hydrogels. Soft and hard substrates were generated with approximate elastic moduli of 1600 and 34,000 Pascals (Pa) respectively. Our results indicate that dermal fibroblasts successfully complete cytokinesis on hard substrates, whereas on soft substrates, a significant number fail and become binucleated. Cytokinesis failure occurs at a step following the formation of the intercellular bridge connecting presumptive daughter cells, suggesting a defect in abscission. Like dermal fibroblasts, mesenchymal stem cells require cell-matrix adhesion for successful cytokinesis. However, in contrast to dermal fibroblasts, they are able to complete cytokinesis on both hard and soft substrates. These results indicate that matrix stiffness regulates the successful completion of cytokinesis, and does so in a cell-type specific manner. To our knowledge, our study is the first to demonstrate that matrix stiffness can affect cytokinesis. Understanding the cell-type specific contribution of matrix compliance to the regulation of cytokinesis will provide new insights important for development, as well as tissue homeostasis and regeneration. |
| اللغة: | English |
| تدمد: | 2046-6390 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9de11ca448edb5739ac4d76a8ed7595 http://bio.biologists.org/content/4/7/885 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a9de11ca448edb5739ac4d76a8ed7595 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20466390 |
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