Regulation of biofilm aging and dispersal in Bacillus subtilis by the alternative sigma factor SigB
| العنوان: | Regulation of biofilm aging and dispersal in Bacillus subtilis by the alternative sigma factor SigB |
|---|---|
| المؤلفون: | Uwe Völker, Federico Argañaraz, Carlos Bauman, Sebastián Cogliati, Liliana Rateni, Cecilia Leñini, Dario German Vileta, Juan Manuel Villalba, Leif Steil, Marco Bartolini, Roberto Grau, Marcos Francisco |
| المصدر: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| بيانات النشر: | American Society for Microbiology, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Motility, Sigma Factor, Bacillus subtilis, Biology, BIOFILM DISPERSAL, Microbiology, Ciencias Biológicas, purl.org/becyt/ford/1 [https], 03 medical and health sciences, Bacterial Proteins, Biología Celular, Microbiología, Sigma factor, purl.org/becyt/ford/1.6 [https], Molecular Biology, Transcription factor, 030304 developmental biology, 0303 health sciences, BACILLUS SUBTILIS, 030306 microbiology, Biofilm, Gene Expression Regulation, Bacterial, biochemical phenomena, metabolism, and nutrition, SIGMA B, biology.organism_classification, Culture Media, Cell biology, Metabolism, Biofilms, STRESS ACTIVATION, bacteria, Biological dispersal, Adaptation, BIOFILM AGING, Locomotion, Bacteria, CIENCIAS NATURALES Y EXACTAS, Research Article |
| الوصف: | Bacterial biofilms are important in natural settings, biotechnology, and medicine. However, regulation of biofilm development and its persistence in different niches is complex and only partially understood. One key step during the biofilm life cycle is dispersal, when motile cells abandon the mature biofilm to spread out and colonize new niches. Here, we show that in the model bacterium Bacillus subtilis the general stress transcription factor SigB is essential for halting detrimental overgrowth of mature biofilm and for triggering dispersal when nutrients become limited. Specifically, SigB-deficient biofilms were larger than wild-type biofilms but exhibited accelerated cell death, significantly greater sensitivity to different stresses, and reduced dispersal. Interestingly, the signal detected by SigB to limit biofilm growth was transduced through the RsbP-dependent metabolic arm of the SigB regulatory cascade, which in turn positively controlled expression of SinR, the master regulator of biofilm formation and cell motility. This novel SigB-SinR regulatory circuit might be important in controlling the fitness of biofilms (either beneficial or harmful) in diverse environments. IMPORTANCE Biofilms are crucial for bacterial survival, adaptation, and dissemination in natural, industrial, and medical systems. Sessile cells embedded in the self-produced extracellular matrix of the biofilm benefit from a division of labor and are protected from environmental insults. However, as the biofilm ages, cells become stressed because of overcrowding, starvation, and accumulation of waste products. How does the sessile biofilm community sense and respond to stressful conditions? Here, we show that in Bacillus subtilis, the transcription factors SigB and SinR control whether cells remain in or leave a biofilm when metabolic conditions become unfavorable. This novel SigB-SinR regulatory circuit might be important for controlling the fitness of biofilms (either beneficial or harmful) in diverse environments. Fil: Bartolini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina Fil: Cogliati, Sebastian Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina Fil: Vileta, Dario German. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina Fil: Bauman, C.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina Fil: Rateni, Liliana Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina Fil: Leñini, Cecilia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina Fil: Argañaraz, F.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina Fil: Francisco, M.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina Fil: Villalba, J.M.. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina Fil: Steil, L.. University Medicine Greifswald. Department of Functional Genomics. Interfaculty Institute for Genetics and Functional Genomics; Alemania Fil: Völker, U.. University Medicine Greifswald; Alemania Fil: Grau, Roberto Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario; Argentina |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a975e8c08cfce1f65fb090b010b2decc https://jb.asm.org/content/201/2/e00473-18 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a975e8c08cfce1f65fb090b010b2decc |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |