Allergens Induce the Release of Lactoferrin by Neutrophils from Asthmatic Patients
| العنوان: | Allergens Induce the Release of Lactoferrin by Neutrophils from Asthmatic Patients |
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| المؤلفون: | Inmaculada Ventura, Rocío Aroca, M Prados, Javier Monteseirín, Bobadilla P, Antonio Vega-Rioja, Ricardo Palacios, Cristina Chamorro, Lourdes Fernández-Delgado, David Rodriguez |
| المساهمون: | [Fernández-Delgado,L, Vega-Rioja,A, Ventura,I, Chamorro,C, Aroca,R, Prados,M, Monteseirín,J] Unidad de Gestión Clínica de Alergia Intercentros, Hospital Universitario Virgen Macarena, Sevilla, Spain. [Monteseirín,J] Departamento de Medicina. Facultad de Medicina. Universidad de Sevilla, Sevilla, Spain. [Bobadilla,P] Servicio de Alergia, Hospital Infanta Elena, Badajoz, Spain. [Rodríguez,D, Palacios,R] Laboratorios Diater, Madrid, Spain., This work was supported by the FIS-Thematic Networks and Co-Operative Research Centres RIRAAF (RD07-0064) and Fundación Alergol, Spain. Javier Monteseirín is under the Programa de Intensificación de la Actividad Investigadora del Sistema Nacional de Salud. |
| المصدر: | PLoS ONE, Vol 10, Iss 10, p e0141278 (2015) PLoS ONE Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid Consejería de Sanidad de la Comunidad de Madrid |
| بيانات النشر: | Public Library of Science (PLoS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Allergy, Galectina 3, Neutrophils, Galectin 3, lcsh:Medicine, medicine.disease_cause, Immunoglobulin E, Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], Allergen, Alérgenos, Phenomena and Processes::Biological Phenomena::Biological Processes [Medical Subject Headings], Receptor, lcsh:Science, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin E [Medical Subject Headings], Multidisciplinary, biology, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Lectins::Galectins::Galectin 3 [Medical Subject Headings], Lactoferrin, food and beverages, Middle Aged, Female, medicine.symptom, Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Physiological Effects of Drugs::Immunologic Factors::Immunosuppressive Agents [Medical Subject Headings], Research Article, Adult, Adolescent, Inflammation, Diseases::Respiratory Tract Diseases::Lung Diseases::Lung Diseases, Obstructive::Asthma [Medical Subject Headings], Young Adult, medicine, Humans, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Antibodies, Monoclonal [Medical Subject Headings], Asma, Asthma, Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Peptide Hydrolases::Endopeptidases::Serine Endopeptidases::Lactoferrin [Medical Subject Headings], business.industry, Receptors, IgE, lcsh:R, Allergens, medicine.disease, Inmunosupresores, In vitro, Case-Control Studies, Anticuerpos monoclonales, Immunology, biology.protein, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Receptors, Cell Surface::Receptors, Immunologic::Receptors, Fc::Receptors, IgE [Medical Subject Headings], lcsh:Q, Chemicals and Drugs::Biological Factors::Antigens::Allergens [Medical Subject Headings], Anatomy::Cells::Blood Cells::Leukocytes::Granulocytes::Neutrophils [Medical Subject Headings], business, Chemicals and Drugs::Biological Factors::Inflammation Mediators [Medical Subject Headings] |
| الوصف: | Journal Article; BACKGROUND Despite the evidence that Lactoferrin (Lf) is involved in allergic asthma processes, it is unknown whether neutrophils can be one of the main cellular sources of this key inflammatory mediator directly in response of an IgE mediated stimulus. The present study was undertaken to analyze this question. METHODS Neutrophils from healthy subjects (n = 34) and neutrophils from allergic asthmatic patients (n = 102) were challenged in vitro with specific allergens to which the patients were sensitized, PAF, or agonist mAbs against IgE-receptors, and the levels of Lf were measured in the culture supernatant. The levels of serum IgE together with the severity of symptoms were also analyzed. RESULTS Lf was released into the culture supernatant of neutrophils from allergic asthmatic patients in response to allergens and PAF. This response was highly allergen-specific, and did not happen in neutrophils from healthy donors. Allergen effect was mimicked by Abs against FcεRI and galectin-3 but not by FcεRII. The levels of released Lf correlated well with the levels of serum specific IgE and severity of asthma symptoms. These observations represent a novel view of neutrophils as an important source of Lf in allergic asthma. Importantly, the levels of released Lf by neutrophils could therefore be used to evaluate disease severity in allergic asthmatic patients. Yes |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a92a92cee8b228a7cc00d6aa6cb039b9 http://europepmc.org/articles/PMC4619071?pdf=render |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a92a92cee8b228a7cc00d6aa6cb039b9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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