Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers
| العنوان: | Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers |
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| المؤلفون: | Gisselle Moraima Chávez-Andrade, Juliane Maria Guerreiro-Tanomaru, Mário Tanomaru-Filho, E. M. Rodrigues, Kennia Scapin Viola, Roberta Bosso-Martelo, Fernanda Ferrari Esteves Torres, Marcela Borsatto Queiroz |
| المساهمون: | Universidade Estadual Paulista (Unesp), Universidade Federal da Bahia (UFBA) |
| المصدر: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Neutral red, Materials science, Dental materials, Radiodensity, Dental Cements, 02 engineering and technology, Enterococcus faecalis, Endodontics, Root Canal Filling Materials, 03 medical and health sciences, chemistry.chemical_compound, Biological properties, 0302 clinical medicine, Anti-Infective Agents, Materials Testing, General Materials Science, Solubility, General Dentistry, Chemical properties, Physical properties, biology, Silicates, fungi, Oxides, 030206 dentistry, Calcium Compounds, 021001 nanoscience & nanotechnology, biology.organism_classification, Staining, Anti-Bacterial Agents, Calcium silicate, Drug Combinations, chemistry, Mechanics of Materials, Alkaline phosphatase, 0210 nano-technology, Antibacterial activity, Nuclear chemistry |
| الوصف: | Made available in DSpace on 2021-06-25T10:18:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-02-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective: To evaluate tricalcium silicate-based (TCS) experimental materials, associated with zirconium oxide (ZrO2), calcium tungstate (CaWO4) or niobium oxide (Nb2O5) radiopacifiers, in comparison with MTA Repair HP (Angelus). Methods: Physicochemical tests: setting time, radiopacity, pH and solubility. In vitro assays: cytotoxicity: MTT and Neutral Red – NR; cell bioactivity: alkaline phosphatase activity (ALP), Alzarin red staining (ARS) and real time PCR (qPCR). Antibacterial activity: direct contact on Enterococcus faecalis in the planktonic form. Physicochemical and ARS data were submitted to ANOVA/Tukey tests; antibacterial activity, to Kruskall–Wallis and Dunn tests; MTT, NR, ALP and qPCR were analyzed by ANOVA/Bonferroni tests (α = 0.05). Results: TCS + CaWO4 presented the longest setting time and MTA HP the shortest. Except for TCS, all the materials presented radiopacity above 3 mmAl. The cements had alkaline pH, antibacterial activity, low solubility and no cytotoxic effects. The highest ALP activity occurred in 14 days, especially to TCS, TCS + ZrO2 and TCS + CaWO4. TCS + ZrO2, TCS + Nb2O5 and MTAHP had higher mineralized nodule formation than those of the negative control (NC). After 7 days, there was no difference in mRNA expression for ALP, when compared to NC. However, after 14 days there was no overexpressed ALP mRNA, especially TCS + Nb2O5, in relation to the CN. All the materials presented antimicrobial action. Significance: The pure tricalcium silicate associated with ZrO2, CaWO4 or Nb2O5 had appropriate physicochemical properties, antibacterial activity, cytocompatibility and induced mineralization in Saos-2, indicating their use as reparative materials. Department of Restorative Dentistry São Paulo State University (UNESP) School of Dentistry Department of Dentistry Bahia Federal University (UFBA) School of Dentistry Department of Restorative Dentistry São Paulo State University (UNESP) School of Dentistry |
| تدمد: | 1879-0097 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8b31728e50f10278bdcb98768d060f9 https://pubmed.ncbi.nlm.nih.gov/33323301 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a8b31728e50f10278bdcb98768d060f9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18790097 |
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