Genetic micro-heterogeneity of Leishmania major in emerging foci of zoonotic cutaneous leishmaniasis in Tunisia
| العنوان: | Genetic micro-heterogeneity of Leishmania major in emerging foci of zoonotic cutaneous leishmaniasis in Tunisia |
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| المؤلفون: | Carola Schweynoch, Hanène Attia, Gabriele Schönian, Rabiaa M. Sghaier, Ghada Mkannez, Sadok Chlif, Tesfaye Gelanew, Dhafer Laouini, Koussay Dellagi, Fatma Z. Guerfali, Aymen Bali, Nabil Belhaj-Hamida |
| المساهمون: | Université de Tunis El Manar (UTM), Laboratoire de Transmission, Contrôle et Immunobiologie des Infections - Laboratory of Transmission, Control and Immunobiology of Infection (LR11IPT02), Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Laboratoire d'Epidémiologie Médicale, Institut Pasteur de Tunis, This work was supported by an NIH/NIAID/DMID Grant Number 5P50AI074178 and the Tunisian Ministry of Higher Education and Research (LR11 IPT02) for KD and DL., We are grateful to Prof. A. Ben Salah and Mr. A. Zaatour for kindly providing us with L. major isolates from the field. We thank Ms. R. Yazidi and Ms. S. Chaâbane for their valuable technical help. We are thankful to Dr. K. Ben-Aissa for her English and grammar corrections and to Dr. J Bettaieb for her advices on statistical analyses. |
| المصدر: | Infection, Genetics and Evolution Infection, Genetics and Evolution, Elsevier, 2016, 43, pp.179-185. ⟨10.1016/j.meegid.2016.04.032⟩ |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, MESH: Leishmaniasis, Cutaneous/transmission, MESH: Leishmania major/genetics, Endemic Diseases, MESH: Tunisia/epidemiology, MESH: Genome, Protozoan, Zoonoses, Leishmania major, MESH: Animals, Phylogeny, MESH: Phylogeny, Genetics, Phylogenetic tree, MESH: Genetic Heterogeneity, 3. Good health, Emerging focus, MESH: Endemic Diseases, MESH: Leishmania major/classification, Infectious Diseases, MESH: Multilocus Sequence Typing, Parasitic disease, Genetic structure, Microsatellite, MESH: Zoonoses, Microbiology (medical), Tunisia, MESH: Psychodidae/parasitology, MESH: Leishmaniasis, Cutaneous/epidemiology, Leishmaniasis, Cutaneous, Locus (genetics), Biology, MESH: DNA, Protozoan/genetics, Microbiology, MESH: Genetic Loci, 03 medical and health sciences, Genetic Heterogeneity, medicine, Animals, Humans, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Typing, Allele, Molecular Biology, MESH: Life Cycle Stages/genetics, Ecology, Evolution, Behavior and Systematics, Alleles, Multi-locus microsatellite typing, MESH: Leishmaniasis, Cutaneous/parasitology, Life Cycle Stages, MESH: Humans, MESH: Alleles, DNA, Protozoan, biology.organism_classification, medicine.disease, MESH: Leishmania major/isolation & purification, 030104 developmental biology, Genetic Loci, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Old focus, MESH: Microsatellite Repeats, Heterogeneity, Psychodidae, MESH: Leishmania major/growth & development, Genome, Protozoan, Zoonotic cutaneous leishmaniasis, Microsatellite Repeats, Multilocus Sequence Typing |
| الوصف: | International audience; Tunisia is endemic for zoonotic cutaneous leishmaniasis (ZCL), a parasitic disease caused by Leishmania (L.) major. ZCL displays a wide clinical polymorphism, with severe forms present more frequently in emerging foci where naive populations are dominant. In this study, we applied the multi-locus microsatellite typing (MLMT) using ten highly informative and discriminative markers to investigate the genetic structure of 35 Tunisian Leishmania (L.) major isolates collected from patients living in five different foci of Central Tunisia (two old and three emerging foci). Phylogenetic reconstructions based on genetic distances showed that nine of the ten tested loci were homogeneous in all isolates with homozygous alleles, whereas one locus (71AT) had a 58/64-bp bi-allelic profile with an allele linked to emerging foci. Promastigote-stage parasites with the 58-bp allele tend to be more resistant to in vitro complement lysis. These results, which stress the geographical dependence of the genetic micro-heterogeneity, may improve our understanding of the ZCL epidemiology and clinical outcome. |
| تدمد: | 1567-7257 1567-1348 |
| DOI: | 10.1016/j.meegid.2016.04.032⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a75392376ae616186931bc55a77a7cd1 https://pubmed.ncbi.nlm.nih.gov/27137082 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a75392376ae616186931bc55a77a7cd1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15677257 15671348 |
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| DOI: | 10.1016/j.meegid.2016.04.032⟩ |