Raptor and rictor expression in patients with human papillomavirus-related oropharyngeal squamous cell carcinoma
| العنوان: | Raptor and rictor expression in patients with human papillomavirus-related oropharyngeal squamous cell carcinoma |
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| المؤلفون: | Hiroyuki Maeda, Jin Uezato, Asanori Kiyuna, Shunsuke Kondo, Hitoshi Hirakawa, Takayuki Uehara, Shinya Agena, Noritomo Kise, Mikio Suzuki, Taro Ikegami, Yukashi Yamashita, Akira Gahana, Narumi Hasegawa, Hidetoshi Kinjyo, Katsunori Tanaka |
| المصدر: | BMC Cancer, Vol 21, Iss 1, Pp 1-14 (2021) BMC Cancer |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Temsirolimus, Cancer Research, Apoptosis, 0302 clinical medicine, Japan, Cell Movement, Surgical oncology, Tumor Cells, Cultured, Overall survival, Papillomaviridae, Oropharyngeal cancer, biology, Cell Cycle, Middle Aged, Prognosis, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Raptor, Gene Expression Regulation, Neoplastic, Survival Rate, Oropharyngeal Neoplasms, Oncology, Rapalog, 030220 oncology & carcinogenesis, Carcinoma, Squamous Cell, mTOR, Female, Research Article, medicine.drug, Human papillomavirus, lcsh:RC254-282, Rictor, 03 medical and health sciences, Biomarkers, Tumor, Genetics, medicine, Humans, In patient, Mechanistic target of rapamycin, PI3K/AKT/mTOR pathway, Cell Proliferation, Papillomavirus Infections, Head and neck cancer, Cancer, Regulatory-Associated Protein of mTOR, medicine.disease, Rapamycin-Insensitive Companion of mTOR Protein, 030104 developmental biology, Cancer research, biology.protein |
| الوصف: | Background Despite reports of a link between human papillomavirus (HPV) infection and mechanistic target of rapamycin (mTOR) signaling activation, the role of the mTOR pathway, especially raptor and rictor, in HPV-related head and neck cancer is still unclear. The aim of the present study was to elucidate the role of the mTOR pathway in HPV-related oropharyngeal squamous cell carcinoma (OPSCC). Methods The present study involved two strategies. The first was to investigate the activity of mTOR and mTOR-related complexes in high-risk HPV-positive (UM-SCC47 and CaSki) and HPV-negative (SCC-4 and SAS) cancer cell lines. The second was to elucidate mTOR complex expression in 80 oropharyngeal cancer tissues and to examine the relationship between mTOR complex expression and survival in patients with OPSCC. Results The UM-SCC47 and CaSki cell lines showed high gene and protein expression of raptor. They also exhibited G1/S and G2/M phase cell cycle arrest following 24 h incubation with 6 μM temsirolimus, a rapamycin analog, and temsirolimus administration inhibited their growth. HPV-related OPSCC samples showed high gene and protein expression of raptor and rictor compared with HPV-unrelated OPSCC. In addition, HPV-related OPSCC patients with high raptor and rictor expression tended to have a worse prognosis than those with low or medium expression. Conclusions These results suggest that raptor and rictor have important roles in HPV-related OPSCC and that temsirolimus is a potential therapeutic agent for patients with HPV-related OPSCC. This is the first report to reveal the overexpression of raptor and rictor in HPV-related OPSCC. |
| اللغة: | English |
| تدمد: | 1471-2407 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6fa06bb03cb0696e24a1a667000561d https://doaj.org/article/2e10d0e7c4a8491e86777c93a40406c9 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a6fa06bb03cb0696e24a1a667000561d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712407 |
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