In vitroevaluation of nanosized carbonate-substituted hydroxyapatite and its polyhydroxyethylmethacrylate nanocomposite
| العنوان: | In vitroevaluation of nanosized carbonate-substituted hydroxyapatite and its polyhydroxyethylmethacrylate nanocomposite |
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| المؤلفون: | Serena M. Best, Roger A. Brooks, William Bonfield, J. Huang, Neil Rushton |
| المصدر: | Journal of Biomedical Materials Research Part A. :598-607 |
| بيانات النشر: | Wiley, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Materials science, Simulated body fluid, Carbonates, Biomedical Engineering, Biocompatible Materials, Apatite, Nanocomposites, Biomaterials, chemistry.chemical_compound, Humans, Composite material, Lactate Dehydrogenases, Cells, Cultured, Polyhydroxyethyl Methacrylate, Osteoblasts, Nanocomposite, Tumor Necrosis Factor-alpha, Macrophages, Metals and Alloys, Hydrogels, Adhesion, Polyhydroxyethylmethacrylate, Durapatite, Solubility, chemistry, Chemical engineering, Transmission electron microscopy, visual_art, Ceramics and Composites, visual_art.visual_art_medium, Nanoparticles, Carbonate, Particle size |
| الوصف: | Nanometer scale carbonate-substituted hydroxyapatite (nanoCHA) particles were prepared and examined using transmission electron microscopy, which revealed their polycrystalline nature with a rod-like morphology (20-30 nm in width and 50-80 nm in length). In vitro cytotoxicity study showed that there was some evidence of lactate dehydrogenase (LDH) release when macrophages were in contact with high concentrations of nanoCHA particles. The levels of LDH release decreased significantly with a reduction in nanoCHA concentration. A similar trend was observed for the inflammatory cytokine TNF-alpha. nanoCHA particles with high carbonate content induced a high level of TNF-a release. Biological testing using a human osteoblast (HOB) cell model found that HOB cells were able to grow and proliferate on a nanoCHA deposited surface. Well organized actin fibers were observed for HOB cells in contact with nanoCHA particles with low carbonate content and the cell proliferation rate was higher on these particles in comparison with those of high carbonate nanoCHA particles. Therefore, low carbonate nanoCHA particles were incorporated into poly-(2-hydroxyethylmethacrylate) matrix to make a nanocomposite. It was found that the nanoCHA composite was hydrophilic and became rubber-like after hydration. Both 20 wt % and 40 wt % composites were able to induce the formation of bone-like apatite after immersion in simulated body fluid. A high bioactivity of the composite was obtained with high loading of the nanoCHA filler. These results demonstrate the potential of formulating nanocomposites for biomedical applications. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 87A: 598-607, 2008 |
| تدمد: | 1552-4965 1549-3296 |
| DOI: | 10.1002/jbm.a.31815 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a50a2e01891bbf77fc3decba7e735a57 https://doi.org/10.1002/jbm.a.31815 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....a50a2e01891bbf77fc3decba7e735a57 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15524965 15493296 |
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| DOI: | 10.1002/jbm.a.31815 |