Intestinal parasite infections in a rural community of Rio de Janeiro (Brazil): Prevalence and genetic diversity of Blastocystis subtypes
| العنوان: | Intestinal parasite infections in a rural community of Rio de Janeiro (Brazil): Prevalence and genetic diversity of Blastocystis subtypes |
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| المؤلفون: | Heloisa Werneck de Macedo, Fernando Campos Sodré, Claudia M. d’Avila-Levy, Ricardo Pereira Igreja, Carolina Valença Barbosa, Rosemary de Jesus Andrade, Helena Santos, Magali G. M. Barreto, José Mauro Peralta |
| المصدر: | PLoS ONE PLoS ONE, Vol 13, Iss 3, p e0193860 (2018) |
| بيانات النشر: | Public Library of Science (PLoS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, Rural Population, 0301 basic medicine, Veterinary medicine, Helminthiasis, lcsh:Medicine, Artificial Gene Amplification and Extension, Blastocystis Infections, medicine.disease_cause, Polymerase Chain Reaction, Ribotyping, Geographical locations, Feces, Intestinal Parasites, 0302 clinical medicine, Medicine and Health Sciences, Prevalence, Pentatrichomonas hominis, Intestinal Diseases, Parasitic, lcsh:Science, Child, Phylogeny, Data Management, Protozoans, Aged, 80 and over, Multidisciplinary, biology, Eukaryota, Phylogenetic Analysis, Middle Aged, 030108 mycology & parasitology, Phylogenetics, Child, Preschool, Female, Ascaris lumbricoides, Brazil, Research Article, Adult, Computer and Information Sciences, Adolescent, 030231 tropical medicine, Intestinal parasite, Research and Analysis Methods, Strongyloides stercoralis, Entamoeba Histolytica, Young Adult, 03 medical and health sciences, Sequence Homology, Nucleic Acid, Protozoan infection, parasitic diseases, Parasitic Diseases, medicine, Animals, Humans, Helminths, Evolutionary Systematics, Molecular Biology Techniques, Molecular Biology, Taxonomy, Aged, Evolutionary Biology, Blastocystis, Protozoan Infections, Base Sequence, lcsh:R, Organisms, Biology and Life Sciences, Genetic Variation, Sequence Analysis, DNA, South America, DNA, Protozoan, biology.organism_classification, medicine.disease, Parasitic Protozoans, Cross-Sectional Studies, Trichuris trichiura, lcsh:Q, Parasitology, People and places, Sequence Alignment |
| الوصف: | Background Intestinal parasitic infections are considered a serious public health problem and widely distributed worldwide, mainly in urban and rural environments of tropical and subtropical countries. Globally, soil-transmitted helminths and protozoa are the most common intestinal parasites. Blastocystis sp. is a highly prevalent suspected pathogenic protozoan, and considered an unusual protist due to its significant genetic diversity and host plasticity. Methodology/main findings A total of 294 stool samples were collected from inhabitants of three rural valleys in Rio de Janeiro, Brazil. The stool samples were evaluated by parasitological methods, fecal culture, nested PCR and PCR/Sequencing. Overall prevalence by parasitological analyses was 64.3% (189 out of 294 cases). Blastocystis sp. (55.8%) was the most prevalent, followed by Endolimax nana (18.7%), Entamoeba histolytica complex (7.1%), hookworm infection (7.1%), Entomoeba coli (5.8%), Giardia intestinalis (4.1%), Iodamoeba butchilii (1.0%), Trichuris trichiura (1.0%), Pentatrichomonas hominis (0.7%), Enterobius vermicularis (0.7%), Ascaris lumbricoides (0.7%) and Strongyloides stercoralis (0.7%). Prevalence of IPIs was significantly different by gender. Phylogenetic analysis of Blastocystis sp. and BLAST search revealed five different subtypes: ST3 (34.0%), ST1 (27.0%), ST2 (27.0%), ST4 (3.5%), ST8 (7.0%) and a non-identified subtype. Conclusions/significance Our findings demonstrate that intestinal parasite infection rates in rural areas of the Sumidouro municipality of Rio de Janeiro, Brazil are still high and remain a challenge to public health. Moreover, our data reveals significant genetic heterogeneity of Blastocystis sp. subtypes and a possible novel subtype, whose confirmation will require additional data. Our study contributes to the understanding of potential routes of transmission, epidemiology, and genetic diversity of Blastocystis sp. in rural areas both at a regional and global scale. |
| تدمد: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0193860 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4eb78afd0d532c0e2ebbf64c46a4cb4 https://doi.org/10.1371/journal.pone.0193860 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a4eb78afd0d532c0e2ebbf64c46a4cb4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0193860 |