Three-dimensional printed polymeric system to encapsulate human mesenchymal stem cells differentiated into islet-like insulin-producing aggregates for diabetes treatment
| العنوان: | Three-dimensional printed polymeric system to encapsulate human mesenchymal stem cells differentiated into islet-like insulin-producing aggregates for diabetes treatment |
|---|---|
| المؤلفون: | Usha Thekkedath, Solmaz F. Afshar, Carly S. Filgueira, Omaima M. Sabek, A. Osama Gaber, Marco Farina, Andrea Ballerini, Alessandro Grattoni, Daniel W. Fraga |
| المصدر: | Journal of Tissue Engineering, Vol 7 (2016) Journal of Tissue Engineering |
| بيانات النشر: | SAGE Publications, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, endocrine system, Scaffold, three-dimensional printer, medicine.medical_treatment, Biomedical Engineering, Medicine (miscellaneous), Clinical uses of mesenchymal stem cells, scaffold, Diabetes treatment, lcsh:Biochemistry, Biomaterials, 03 medical and health sciences, Human bone marrow, Diabetes mellitus, medicine, lcsh:QD415-436, polylactic acid, mesenchymal stem cells, geography, geography.geographical_feature_category, diabetes, business.industry, Insulin, Mesenchymal stem cell, Anatomy, Islet, medicine.disease, 030104 developmental biology, medicine.anatomical_structure, Cancer research, Original Article, business, Pancreas |
| الوصف: | Diabetes is one of the most prevalent, costly, and debilitating diseases in the world. Pancreas and islet transplants have shown success in re-establishing glucose control and reversing diabetic complications. However, both are limited by donor availability, need for continuous immunosuppression, loss of transplanted tissue due to dispersion, and lack of vascularization. To overcome the limitations of poor islet availability, here, we investigate the potential of bone marrow–derived mesenchymal stem cells differentiated into islet-like insulin-producing aggregates. Islet-like insulin-producing aggregates, characterized by gene expression, are shown to be similar to pancreatic islets and display positive immunostaining for insulin and glucagon. To address the limits of current encapsulation systems, we developed a novel three-dimensional printed, scalable, and potentially refillable polymeric construct (nanogland) to support islet-like insulin-producing aggregates’ survival and function in the host body. In vitro studies showed that encapsulated islet-like insulin-producing aggregates maintained viability and function, producing steady levels of insulin for at least 4 weeks. Nanogland—islet-like insulin-producing aggregate technology here investigated as a proof of concept holds potential as an effective and innovative approach for diabetes cell therapy. |
| تدمد: | 2041-7314 |
| DOI: | 10.1177/2041731416638198 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a484c0df324de3afdeb97d73530a70ee https://doi.org/10.1177/2041731416638198 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a484c0df324de3afdeb97d73530a70ee |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20417314 |
|---|---|
| DOI: | 10.1177/2041731416638198 |