التفاصيل البيبلوغرافية
| العنوان: |
c(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD |
| المؤلفون: |
Ying-Zheng Zhao, Qing-Hua Lan, Cui-Tao Lu, He-Lin Xu, Bin Chen, Ming-Ling Fang, Qi-Long Wu, Hui Li, Cui Xiong, Jing-Hong Xu, Jin-Hua Cai, Fang-Yi Tao, Shu-Ting Zhu |
| بيانات النشر: |
Taylor & Francis, 2023. |
| سنة النشر: |
2023 |
| مصطلحات موضوعية: |
Surgical resection, Biomedical Engineering, Pharmaceutical Science, Medicine (miscellaneous), Nanoparticle, Poloxamer, macromolecular substances, 02 engineering and technology, Peptides, Cyclic, 03 medical and health sciences, Drug Delivery Systems, 0302 clinical medicine, Cell Line, Tumor, Glioma, Quantum Dots, medicine, Animals, Humans, Fluorescent Dyes, Microbubbles, Chemistry, business.industry, General Medicine, 021001 nanoscience & nanotechnology, medicine.disease, Fluorescence, Rats, nervous system diseases, Disease Models, Animal, Surgery, Computer-Assisted, Ultrasonic Waves, Quantum dot, 030220 oncology & carcinogenesis, Nanoparticles, Administration, Intravenous, 0210 nano-technology, Nuclear medicine, business, Biotechnology, Glioblastoma |
| الوصف: |
Surgical resection remains the preferred approach for some patients with glioblastoma (GBM), and eradication of the residual tumour niche after surgical resection is very helpful for prolonging patient survival. However, complete surgical resection of invasive GBM is difficult because of its ambiguous boundary. Herein, a novel targeting material, c(RGDyk)-poloxamer-188, was synthesized by modifying carboxyl-terminated poloxamer-188 with a glioma-targeting cyclopeptide, c(RGDyk). Quantum dots (QDs) as fluorescent probe were encapsulated into the self-assembled c(RGDyk)-poloxamer-188 polymer nanoparticles (NPs) to construct glioma-targeted QDs-c(RGDyk)NP for imaging-guided surgical resection of GBM. QDs-c(RGDyk)NP exhibited a moderate hydrodynamic diameter of 212.4 nm, a negative zeta potential of –10.1 mV and good stability. QDs-c(RGDyk)NP exhibited significantly lower toxicity against PC12 and C6 cells and HUVECs than free QDs. Moreover, in vitro cellular uptake experiments demonstrated that QDs-c(RGDyk)NP specifically targeted C6 cells, making them display strong fluorescence. Combined with ultrasound-targeted microbubble destruction (UTMD), QDs-c(RGDyk)NP specifically accumulated in glioma tissue in orthotropic tumour rats after intravenous administration, evidenced by ex vivo NIR fluorescence imaging of bulk brain and glioma tissue sections. Furthermore, fluorescence imaging with QDs-c(RGDyk)NP guided accurate surgical resection of glioma. Finally, the safety of QDs-c(RGDyk)NP was verified using pathological HE staining. In conclusion, QDs-c(RGDyk)NP may be a potential imaging probe for imaging-guided surgery. |
| DOI: |
10.6084/m9.figshare.23618622 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3ea20215c0b5d6447b7e280444cfa0d |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi.dedup.....a3ea20215c0b5d6447b7e280444cfa0d |
| قاعدة البيانات: |
OpenAIRE |