Tea2p Is a Kinesin-like Protein Required to Generate Polarized Growth in Fission Yeast
| العنوان: | Tea2p Is a Kinesin-like Protein Required to Generate Polarized Growth in Fission Yeast |
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| المؤلفون: | Lauren Aveline, Juan Mata, Heidi Browning, J. Richard McIntosh, Jacqueline Hayles, Paul Nurse |
| المساهمون: | Mata, Juan [0000-0002-5514-3653], Apollo - University of Cambridge Repository |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | Rockefeller University Press, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Saccharomyces cerevisiae Proteins, Transcription, Genetic, Microtubule-associated protein, Genes, Fungal, Molecular Sequence Data, Saccharomyces cerevisiae, Biology, Microtubules, kinesin, Fungal Proteins, Amino Acid Sequence Cell Compartmentation Cell Polarity/*genetics Cloning, Molecular Fungal Proteins/genetics *Genes, Fungal Microtubule-Associated Proteins/*genetics Microtubules/*ultrastructure Mole, 03 medical and health sciences, 0302 clinical medicine, Microtubule, Schizosaccharomyces, Cell polarity, Amino Acid Sequence, Microtubule end, Cloning, Molecular, Cytoplasmic microtubule, 030304 developmental biology, 0303 health sciences, Sequence Homology, Amino Acid, Molecular Motor Proteins, Cell Polarity, cytoskeleton, Sequence Analysis, DNA, Cell Biology, biology.organism_classification, Cell Compartmentation, Cell biology, Protein Biosynthesis, Schizosaccharomyces pombe, Kinesin, Original Article, Schizosaccharomyces pombe Proteins, Microtubule-Associated Proteins, 030217 neurology & neurosurgery, microtubule |
| الوصف: | Cytoplasmic microtubules are critical for establishing and maintaining cell shape and polarity. Our investigations of kinesin-like proteins (klps) and morphological mutants in the fission yeast Schizosaccharomyces pombe have identified a kinesin-like gene, tea2+, that is required for cells to generate proper polarized growth. Cells deleted for this gene are often bent during exponential growth and initiate growth from improper sites as they exit stationary phase. They have a reduced cytoplasmic microtubule network and display severe morphological defects in genetic backgrounds that produce long cells. The tip-specific marker, Tea1p, is mislocalized in both tea2-1 and tea2Δ cells, indicating that Tea2p function is necessary for proper localization of Tea1p. Tea2p is localized to the tips of the cell and in a punctate pattern within the cell, often coincident with the ends of cytoplasmic microtubules. These results suggest that this kinesin promotes microtubule growth, possibly through interactions with the microtubule end, and that it is important for establishing and maintaining polarized growth along the long axis of the cell. |
| وصف الملف: | application/pdf |
| تدمد: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.151.1.15 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a29ff1d0807ec9642316f112e3403928 https://doi.org/10.1083/jcb.151.1.15 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a29ff1d0807ec9642316f112e3403928 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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| DOI: | 10.1083/jcb.151.1.15 |