Methanogenic Biodegradation of iso-Alkanes by Indigenous Microbes from Two Different Oil Sands Tailings Ponds
| العنوان: | Methanogenic Biodegradation of iso-Alkanes by Indigenous Microbes from Two Different Oil Sands Tailings Ponds |
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| المؤلفون: | Mohd Faidz Mohamad Shahimin, Tariq Siddique, Julia M. Foght |
| المصدر: | Microorganisms, Vol 9, Iss 1569, p 1569 (2021) Microorganisms Volume 9 Issue 8 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), QH301-705.5, Methanogenesis, Oil sands tailings ponds, iso-alkanes, 010501 environmental sciences, 01 natural sciences, Microbiology, Methanosaeta, preferential hydrocarbon biodegradation, 03 medical and health sciences, Virology, Biology (General), 0105 earth and related environmental sciences, biology, Methanoregula, Chemistry, methanogenesis, Biodegradation, biology.organism_classification, Tailings, Peptococcaceae, 030104 developmental biology, Environmental chemistry, oil sands tailings, Oil sands, Microcosm |
| الوصف: | iso-Alkanes, a major fraction of the solvents used in bitumen extraction from oil sand ores, are slow to biodegrade in anaerobic tailings ponds. We investigated methanogenic biodegradation of iso-alkane mixtures comprising either three (2-methylbutane, 2-methylpentane, 3-methylpentane) or five (2-methylbutane, 2-methylpentane, 2-methylhexane, 2-methylheptane, 2-methyloctane) iso-alkanes representing paraffinic and naphtha solvents, respectively. Mature fine tailings (MFT) collected from two tailings ponds, having different residual solvents (paraffinic solvent in Canadian Natural Upgrading Limited (CNUL) and naphtha in Canadian Natural Resources Limited (CNRL)), were amended separately with the two mixtures and incubated in microcosms for ~1600 d. The indigenous microbes in CNUL MFT produced methane from the three-iso-alkane mixture after a lag of ~200 d, completely depleting 2-methylpentane while partially depleting 2-methylbutane and 3-methylpentane. CNRL MFT exhibited a similar degradation pattern for the three iso-alkanes after a lag phase of ~700 d, but required 1200 d before beginning to produce methane from the five-iso-alkane mixture, preferentially depleting components in the order of decreasing carbon chain length. Peptococcaceae members were key iso-alkane-degraders in both CNUL and CNRL MFT but were associated with different archaeal partners. Co-dominance of acetoclastic (Methanosaeta) and hydrogenotrophic (Methanolinea and Methanoregula) methanogens was observed in CNUL MFT during biodegradation of three-iso-alkanes whereas CNRL MFT was enriched in Methanoregula during biodegradation of three-iso-alkanes and in Methanosaeta with five-iso-alkanes. This study highlights the different responses of indigenous methanogenic microbial communities in different oil sands tailings ponds to iso-alkanes. |
| وصف الملف: | application/pdf |
| تدمد: | 2076-2607 |
| DOI: | 10.3390/microorganisms9081569 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a26bc7c4ad40ded80c57e3a3a2ac30ef https://doi.org/10.3390/microorganisms9081569 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a26bc7c4ad40ded80c57e3a3a2ac30ef |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20762607 |
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| DOI: | 10.3390/microorganisms9081569 |