MCP-1 promotes detrimental cardiac physiology, pulmonary edema, and death in thecpkmodel of polycystic kidney disease
| العنوان: | MCP-1 promotes detrimental cardiac physiology, pulmonary edema, and death in thecpkmodel of polycystic kidney disease |
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| المؤلفون: | Timothy A. Fields, Xiaogang Li, Dawson Foster, Xia Zhou, Xiaoyan Li, Katherine I. Swenson-Fields, Julian Vallejo, Jacqueline D. Peda, Reena Rao, Darren P. Wallace, Sally M. Salah, Michael J. Wacker, James D. Meisenheimer |
| المصدر: | Am J Physiol Renal Physiol |
| بيانات النشر: | American Physiological Society, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Time Factors, Physiology, Cardiomegaly, Pulmonary Edema, Kidney, Polycystic kidney disease, Animals, Humans, Medicine, Lung, Cells, Cultured, Chemokine CCL2, Mice, Knockout, Polycystic Kidney Diseases, business.industry, Macrophages, Myocardium, Membrane Proteins, Arrhythmias, Cardiac, Pulmonary edema, medicine.disease, Fibrosis, Cardiovascular physiology, Mice, Inbred C57BL, Disease Models, Animal, medicine.anatomical_structure, Renal cysts, Disease Progression, Inflammation Mediators, business, Research Article |
| الوصف: | Polycystic kidney disease (PKD) is characterized by slowly expanding renal cysts that damage the kidney, typically resulting in renal failure by the fifth decade. The most common cause of death in these patients, however, is cardiovascular disease. Expanding cysts in PKD induce chronic kidney injury that is accompanied by immune cell infiltration, including macrophages, which we and others have shown can promote disease progression in PKD mouse models. Here, we show that monocyte chemoattractant protein-1 [MCP-1/chemokine (C-C motif) ligand 2 (CCL2)] is responsible for the majority of monocyte chemoattractant activity produced by renal PKD cells from both mice and humans. To test whether the absence of MCP-1 lowers renal macrophage concentration and slows disease progression, we generated genetic knockout (KO) of MCP-1 in a mouse model of PKD [congenital polycystic kidney ( cpk) mice]. Cpk mice are born with rapidly expanding renal cysts, accompanied by a decline in kidney function and death by postnatal day 21. Here, we report that KO of MCP-1 in these mice increased survival, with some mice living past 3 mo. Surprisingly, however, there was no significant difference in renal macrophage concentration, nor was there improvement in cystic disease or kidney function. Examination of mice revealed cardiac hypertrophy in cpk mice, and measurement of cardiac electrical activity via ECG revealed repolarization abnormalities. MCP-1 KO did not affect the number of cardiac macrophages, nor did it alleviate the cardiac aberrancies. However, MCP-1 KO did prevent the development of pulmonary edema, which occurred in cpk mice, and promoted decreased resting heart rate and increased heart rate variability in both cpk and noncystic mice. These data suggest that in this mouse model of PKD, MCP-1 altered cardiac/pulmonary function and promoted death outside of its role as a macrophage chemoattractant. |
| تدمد: | 1522-1466 1931-857X |
| DOI: | 10.1152/ajprenal.00240.2018 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a257b701e06104b6b3b5cfeb86de1229 https://doi.org/10.1152/ajprenal.00240.2018 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a257b701e06104b6b3b5cfeb86de1229 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221466 1931857X |
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| DOI: | 10.1152/ajprenal.00240.2018 |