Screening of Microbial Volatile Organic Compounds for Detection of Disease in Cattle: Development of Lab-scale Method
| العنوان: | Screening of Microbial Volatile Organic Compounds for Detection of Disease in Cattle: Development of Lab-scale Method |
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| المؤلفون: | Tyler C. Thacker, Pauline Nol, Jacek A. Koziel, Somchai Rice, Christine K. Ellis, Kurt C. VerCauteren, Devin L. Maurer |
| المصدر: | Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019) Scientific Reports |
| بيانات النشر: | Nature Publishing Group, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Change over time, Microbiological culture, Lab scale, Paratuberculosis, Cattle Diseases, lcsh:Medicine, Disease, Biology, Article, Gas Chromatography-Mass Spectrometry, 03 medical and health sciences, Medical and clinical diagnostics, 0302 clinical medicine, Vaccine strain, medicine, Animals, Humans, lcsh:Science, Ecological epidemiology, Volatile Organic Compounds, Multidisciplinary, Complex matrix, business.industry, Infectious-disease diagnostics, lcsh:R, medicine.disease, Biotechnology, Mycobacterium avium paratuberculosis, Mycobacterium avium subsp. paratuberculosis, 030104 developmental biology, Cattle, lcsh:Q, business, Microbiology techniques, 030217 neurology & neurosurgery, Agroecology, Biomarkers |
| الوصف: | The primary hurdle for diagnosis of some diseases is the long incubation required to culture and confirm the presence of bacteria. The concept of using microbial VOCs as “signature markers” could provide a faster and noninvasive diagnosis. Finding biomarkers is challenging due to the specificity required in complex matrices. The objectives of this study were to (1) build/test a lab-scale platform for screening of microbial VOCs and (2) apply it to Mycobacterium avium paratuberculosis; the vaccine strain of M. bovis Bacillus Calmette-Guérin; and M. kansasii to demonstrate detection times greater those typically required for culture. SPME-GC-MS was used for sampling, sample preparation, and analyses. For objective (1), a testing platform was built for headspace sampling of bacterial cultures grown in standard culture flasks via a biosecure closed-loop circulating airflow system. For (2), results show that the suites of VOCs produced by Mycobacteria ssp. change over time and that individual strains produce different VOCs. The developed method was successful in discriminating between strains using a pooled multi-group analysis, and in timepoint-specific multi- and pair-wise comparisons. The developed testing platform can be useful for minimally invasive and biosecure collection of biomarkers associated with human, wildlife and livestock diseases for development of diagnostic point-of-care and field surveillance. |
| اللغة: | English |
| تدمد: | 2045-2322 |
| DOI: | 10.1038/s41598-019-47907-w |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2250d39724316ea30a97b43616a9db9 http://link.springer.com/article/10.1038/s41598-019-47907-w |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a2250d39724316ea30a97b43616a9db9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-019-47907-w |