Hierarchical integration of mitochondrial and nuclear positioning pathways by the Num1 EF hand
| العنوان: | Hierarchical integration of mitochondrial and nuclear positioning pathways by the Num1 EF hand |
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| المؤلفون: | Heidi L. Anderson, Jason C. Casler, Laura L. Lackner |
| المصدر: | Molecular Biology of the Cell. 33 |
| بيانات النشر: | American Society for Cell Biology (ASCB), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Cell Nucleus, Organelles, Cytoplasm, Saccharomyces cerevisiae Proteins, Dyneins, Biological Transport, Saccharomyces cerevisiae, Spindle Apparatus, Cell Biology, Microtubules, Mitochondria, Cytoskeletal Proteins, EF Hand Motifs, Molecular Biology |
| الوصف: | Positioning organelles at the right place and time is critical for their function and inheritance. In budding yeast, mitochondrial and nuclear positioning require the anchoring of mitochondria and dynein to the cell cortex by clusters of Num1. We have previously shown that mitochondria drive the assembly of cortical Num1 clusters, which then serve as anchoring sites for mitochondria and dynein. When mitochondrial inheritance is inhibited, mitochondrial-driven assembly of Num1 in buds is disrupted and defects in dynein-mediated spindle positioning are observed. Using a structure-function approach to dissect the mechanism of mitochondria-dependent dynein anchoring, we found that the EF hand-like motif (EFLM) of Num1 and its ability to bind calcium are required to bias dynein anchoring on mitochondria-associated Num1 clusters. Consistently, when the EFLM is disrupted, we no longer observe defects in dynein activity following inhibition of mitochondrial inheritance. Thus, the Num1 EFLM functions to bias dynein anchoring and activity in nuclear inheritance subsequent to mitochondrial inheritance. We hypothesize that this hierarchical integration of organelle positioning pathways by the Num1 EFLM contributes to the regulated order of organelle inheritance during the cell cycle. |
| تدمد: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e21-12-0610-t |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a214746fa89e1d61be5f318177fa3fbb https://doi.org/10.1091/mbc.e21-12-0610-t |
| رقم الانضمام: | edsair.doi.dedup.....a214746fa89e1d61be5f318177fa3fbb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
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| DOI: | 10.1091/mbc.e21-12-0610-t |