Adjuvant activity of 6-O-acyl-muramyldipeptides to enhance primary cellular and humoral immune responses in guinea pigs: adaptability to various vehicles and pyrogenicity
| العنوان: | Adjuvant activity of 6-O-acyl-muramyldipeptides to enhance primary cellular and humoral immune responses in guinea pigs: adaptability to various vehicles and pyrogenicity |
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| المؤلفون: | Shoichi Kusumoto, Tetsuo Shiba, Shozo Kotani, S Kanoh, Masahiko Tsujimoto, F Kinoshita |
| المصدر: | Infection and Immunity. 53:511-516 |
| بيانات النشر: | American Society for Microbiology, 1986. |
| سنة النشر: | 1986 |
| مصطلحات موضوعية: | Fever, medicine.medical_treatment, Guinea Pigs, Immunology, Stimulation, Muramic acid, Microbiology, Structure-Activity Relationship, chemistry.chemical_compound, Immune system, Adjuvants, Immunologic, Antigen, parasitic diseases, medicine, Animals, chemistry.chemical_classification, Immunity, Cellular, Liposome, biology, Fatty acid, body regions, Ovalbumin, Infectious Diseases, chemistry, Biochemistry, Antibody Formation, Liposomes, biology.protein, Emulsions, Female, Parasitology, Rabbits, Pharmaceutical Vehicles, Acetylmuramyl-Alanyl-Isoglutamine, Adjuvant, Research Article |
| الوصف: | Thirteen 6-O-acyl-N-acetylmuramyl-L-alanyl-D-isoglutamines (6-O-acyl-MDPs), including four inactive D-isoasparagine and L-isoglutamine analogs, were tested for their pyrogenicity and immunopotentiating activity to stimulate primary humoral and cellular immune responses in guinea pigs to a model protein antigen, ovalbumin, when administered in various vehicles. Among them, derivatives whose muramic acid residue was substituted by alpha-branched (and beta-hydroxylated) higher fatty acids at the carbon-6 position, especially 6-O-(2-tetradecylhexadecanoyl)-MDP (B3O-MDP) and, to a lesser extent, 6-O-(3-hydroxy-2-docosylhexacosanoyl)-MDP (BH48-MDP) and its L-serine analog [BH48-MDP(L-Ser)], were found to exert strong adjuvant activity in both the induction of delayed-type hypersensitivity and the stimulation of circulating precipitating antibody levels when combined with nonirritating vehicles (liposomes, squalene-in-water emulsion, and phosphate-buffered saline). These vehicles did not efficiently support the adjuvant activity of MDP, the parent molecule of the above lipophilic derivatives. Pyrogenicity tests showed that introduction of alpha-branched higher fatty acid groups but not of straight, long-chain fatty acids at the 6-position of the muramic acid residue resulted in marked decrease of the pyrogenicity inherent to MDP via intravenous administration. |
| تدمد: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.53.3.511-516.1986 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1b22ee091ad58f038f4409e6352f686 https://doi.org/10.1128/iai.53.3.511-516.1986 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a1b22ee091ad58f038f4409e6352f686 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985522 00199567 |
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| DOI: | 10.1128/iai.53.3.511-516.1986 |