Testing Anti-Pneumococcal Antibody Function Using Bacteria and Primary Neutrophils
| العنوان: | Testing Anti-Pneumococcal Antibody Function Using Bacteria and Primary Neutrophils |
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| المؤلفون: | Essi Y. I. Tchalla, Manmeet Bhalla, Elsa N. Bou Ghanem, Shaunna R. Simmons |
| المصدر: | Methods Mol Biol Vaccine Delivery Technology ISBN: 9781071607947 |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Neutrophils, Bone Marrow Cells, medicine.disease_cause, Pneumococcal Infections, Article, Microbiology, Flow cytometry, Pneumococcal Vaccines, Mice, Streptococcus pneumoniae, medicine, Animals, Opsonin, Phagocytes, medicine.diagnostic_test, biology, business.industry, Immune Sera, medicine.disease, Flow Cytometry, Antibodies, Bacterial, Vaccination, Antibody opsonization, Pneumococcal infections, Pneumococcal vaccine, biology.protein, Antibody, business, Biomarkers |
| الوصف: | Antibodies against Streptococcus pneumoniae (pneumococcus) following vaccination are crucial for host protection against invasive pneumococcal infections. The antibodies induced by pneumococcal vaccines act as opsonins to mediate bacterial uptake and killing by host phagocytic cells, especially polymorphonuclear leukocytes (PMNs) also called neutrophils. Therefore, it is important to measure not only the levels of antibodies induced by a pneumococcal vaccine candidate but their actual functional capacity in mediating bacterial opsonization and killing by PMNs. Here, we describe a protocol to demonstrate effective deposition of vaccine-induced antibodies on the surface of S. pneumoniae by flow cytometry and subsequent opsonophagocytic killing (OPH) by murine bone-marrow derived PMNs. |
| ردمك: | 978-1-07-160794-7 |
| تدمد: | 1940-6029 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1ab891589387612af9b83328d809d5b https://pubmed.ncbi.nlm.nih.gov/32959268 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a1ab891589387612af9b83328d809d5b |
| قاعدة البيانات: | OpenAIRE |
| ردمك: | 9781071607947 |
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| تدمد: | 19406029 |