Characteristics of Cefotaxime-Resistant Escherichia coli from Wild Birds in The Netherlands
| العنوان: | Characteristics of Cefotaxime-Resistant Escherichia coli from Wild Birds in The Netherlands |
|---|---|
| المؤلفون: | Peter van Tulden, Dik Mevius, Kees Veldman, Joop Testerink, Arie Kant |
| المصدر: | Applied and Environmental Microbiology 79 (2013) 24 Applied and Environmental Microbiology, 79(24), 7556-7561 |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Salmonella, Cefotaxime, Epidemiology, ctx-m, medicine.disease_cause, Polymerase Chain Reaction, Applied Microbiology and Biotechnology, Plasmid, Cloaca, Environmental Microbiology, polycyclic compounds, Escherichia coli Infections, environments, Netherlands, spectrum-beta-lactamase, Ecology, biology, Broth microdilution, Bacteriologie, Bacteriology, Host Pathogen Interaction & Diagnostics, Enterobacteriaceae, Anti-Bacterial Agents, Electrophoresis, Gel, Pulsed-Field, antibiotic-resistance, Biotechnology, medicine.drug, plasmids, Bioinformatica & Diermodellen, salmonella, Microbial Sensitivity Tests, gulls, beta-Lactam Resistance, Microbiology, Birds, Bio-informatics & Animal models, Escherichia coli, medicine, Pulsed-field gel electrophoresis, Animals, Epidemiology, Bio-informatics & Animal models, Typing, Host Pathogen Interaction & Diagnostics, Epidemiologie, Bacteriology, Sequence Analysis, DNA, biochemical phenomena, metabolism, and nutrition, Microarray Analysis, biology.organism_classification, bacterial infections and mycoses, Host Pathogen Interactie & Diagnostiek, Molecular Typing, extended-spectrum, Epidemiologie, Bioinformatica & Diermodellen, Bacteriologie, Host Pathogen Interactie & Diagnostiek, bacteria, CVI - Divisie Bacteriologie en TSE's, natural reserve, Food Science, enterobacteriaceae |
| الوصف: | Cloacal swabs from carcasses of Dutch wild birds obtained in 2010 and 2011 were selectively cultured on media with cefotaxime to screen for the presence of extended-spectrum β-lactamase (ESBL)/AmpC-producing Escherichia coli . Subsequently, all cefotaxime-resistant E. coli isolates were tested by broth microdilution and microarray. The presence of ESBL/AmpC and coexisting plasmid-mediated quinolone resistance (PMQR) genes was confirmed by PCR and sequencing. To determine the size of plasmids and the location of ESBL and PMQR genes, S1 pulsed-field gel electrophoresis (PFGE) was performed on transformants, followed by Southern blot hybridization. The study included 414 cloacal swabs originating from 55 different bird species. Cefotaxime-resistant E. coli isolates were identified in 65 birds (15.7%) from 21 different species. In all, 65 cefotaxime-resistant E. coli ESBL/AmpC genes were detected, mainly comprising variants of bla CTX-M and bla CMY-2 . Furthermore, PMQR genes [ aac(6 ′ )-lb-cr , qnrB1 , and qnrS1 ] coincided in seven cefotaxime-resistant E. coli isolates. Overall, replicon typing of the ESBL/AmpC-carrying plasmids demonstrated the predominant presence of IncI1 ( n = 31) and variants of IncF ( n = 18). Our results indicate a wide dissemination of ESBL and AmpC genes in wild birds from The Netherlands, especially among aquatic-associated species (waterfowl, gulls, and waders). The identified genes and plasmids reflect the genes found predominantly in livestock animals as well as in humans. |
| وصف الملف: | application/pdf; application/octet-stream |
| اللغة: | English |
| تدمد: | 0099-2240 |
| DOI: | 10.1128/aem.01880-13 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0e887700a96e534dc9f78ee8bcaedf4 https://doi.org/10.1128/aem.01880-13 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a0e887700a96e534dc9f78ee8bcaedf4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00992240 |
|---|---|
| DOI: | 10.1128/aem.01880-13 |