Molecular cloning and complete nucleotide sequence of carnation Italian ringspot tombusvirus genomic and defective interfering RNAs
| العنوان: | Molecular cloning and complete nucleotide sequence of carnation Italian ringspot tombusvirus genomic and defective interfering RNAs |
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| المؤلفون: | J. Burgyan, Marcello Russo, Luisa Rubino |
| المصدر: | Archives of Virology. 140:2027-2039 |
| بيانات النشر: | Springer Science and Business Media LLC, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | DNA, Complementary, Tombusvirus, Genes, Viral, Molecular Sequence Data, RNA-dependent RNA polymerase, Molecular cloning, Genome, Open Reading Frames, Viral Proteins, Virology, Cluster Analysis, Cloning, Molecular, DNA Primers, Viral Structural Proteins, Genetics, Base Sequence, Sequence Homology, Amino Acid, biology, Nucleic acid sequence, Defective Viruses, RNA, General Medicine, Plants, biology.organism_classification, Molecular biology, Tombusviridae, Open reading frame, RNA, Viral, Sequence Alignment |
| الوصف: | The complete sequences of genomic and defective interfering (DI) RNAs of carnation Italian ringspot tombusvirus (CIRV) were determined. The genome (4760 nt) has an organization identical to that reported for other tombusviruses except that the pre-readthrough domain of the viral replicase encoded by the 5'-proximal open reading frame (ORF) is larger. In particular, the N-terminal region of this protein differs from the corresponding region of the other members of the genus Tombusvirus and Carmovirus. Two DI RNAs were found in infected tissues whose sequences were completely derived from genomic RNA. The smaller molecule (474 nt) is contained completely within the larger molecule (656 nt), which suggests that it is derived from the larger one. |
| تدمد: | 1432-8798 0304-8608 |
| DOI: | 10.1007/bf01322690 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9fa1433900f42ae1f488a026c4c3c425 https://doi.org/10.1007/bf01322690 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....9fa1433900f42ae1f488a026c4c3c425 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14328798 03048608 |
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| DOI: | 10.1007/bf01322690 |