In vitro storage of plum germplasm by slow growth
| العنوان: | In vitro storage of plum germplasm by slow growth |
|---|---|
| المؤلفون: | Francesco Sottile, S. Gianní |
| المساهمون: | Gianni, S, SOTTILE, Francesco |
| المصدر: | Horticultural Science. 42:61-69 |
| بيانات النشر: | Czech Academy of Agricultural Sciences, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Germplasm, Genotype, In vitro preservation, Plant genetic resources, Prunus, Sucrose, Cold storage, 030105 genetics & heredity, Horticulture, Biology, 01 natural sciences, Prunus cerasifera, Settore AGR/03 - Arboricoltura Generale E Coltivazioni Arboree, 03 medical and health sciences, Prunus, chemistry.chemical_compound, chemistry, Botany, Shoot, Cultivar, 010606 plant biology & botany, Explant culture |
| الوصف: | Gianni S., Sottile F. (2015): In vitro storage of plum germplasm by slow growth . Hort. Sci. (Prague), 42: 61–69. In this study, in vitro slow growth storage was investigated in four cultivars of two Sicilian (Southern Italy) plum species (Prunus domestica L. and Prunus cerasifera Ehrh. – two genotypes each). Established shoot cultures were preserved at 4°C in the dark in a Murashige and Skoog basal medium containing one of two different concentrations of sucrose (20 and 30 g/l) and with or without growth regulators. We tested the effects of cold storage, genotype and media on survival and re-growth capacity of explants after 3, 6, 9 and 12 months of storage. Effective minimal growth under cold conditions occurred in all four genotypes. The media composition did not affect survival, which, instead, appeared to be genotype-dependent. P. domestica genotypes survived cold storage the longest, for 12 and 9 months; instead, P. cerasifera ones remained viable for up to 6 months. All genotypes retained proliferation capacity under standard conditions and their re-growth capacity seems to be strongly genotype-dependent, closely related to their individual performance in response to the experimental condition of storage. |
| تدمد: | 1805-9333 0862-867X |
| DOI: | 10.17221/186/2014-hortsci |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f7e4bdcc6c0155fba1e13ded8981b2c https://doi.org/10.17221/186/2014-hortsci |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9f7e4bdcc6c0155fba1e13ded8981b2c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18059333 0862867X |
|---|---|
| DOI: | 10.17221/186/2014-hortsci |