SUMOylation stabilizes sister kinetochore biorientation to allow timely anaphase
| العنوان: | SUMOylation stabilizes sister kinetochore biorientation to allow timely anaphase |
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| المؤلفون: | Dean Clift, Adele L. Marston, Ronald T. Hay, Yehui Wu, Olga O. Nerusheva, A. Arockia Jeyaprakash, Menglu Wang, Zuzana Storchova, David A. Kelly, Andreas U. Wallek, Juri Rappsilber, Claudia Schaffner, Xue Bessie Su, Michael H. Tatham, Christos Spanos |
| المصدر: | The Journal of Cell Biology Su, B, Wang, M, Schaffner, C, Nerusheva, O O, Clift, D, Spanos, C, Kelly, D, Tatham, M H, Wallek, A, Wu, Y, Rappsilber, J, Jeyaprakash, A A, Storchova, Z, Hay, R T & Marston, A L 2021, ' SUMOylation stabilizes sister kinetochore biorientation to allow timely anaphase ', Journal of Cell Biology, vol. 220, no. 7, e202005130 . https://doi.org/10.1083/jcb.202005130 |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Protein sumoylation, Saccharomyces cerevisiae Proteins, Ubiquitin-Protein Ligases, Biorientation, Mitosis, Saccharomyces cerevisiae, Spindle Apparatus, Biology, Chromatids, Article, 03 medical and health sciences, 0302 clinical medicine, Chromosome Segregation, Centromere, Genetics, Sister chromatids, Humans, Protein Phosphatase 2, Kinetochores, 030304 developmental biology, Anaphase, 0303 health sciences, Kinetochore, Nuclear Proteins, Sumoylation, Cell Biology, Cell biology, Establishment of sister chromatid cohesion, Chromosome passenger complex, Carrier Proteins, 030217 neurology & neurosurgery, Cell Cycle and Division |
| الوصف: | Su et al. show that SUMOylation stabilizes bioriented kinetochore–microtubule attachments in mitosis to allow timely anaphase onset. They identify the shugoshin pericentromeric adaptor protein and the chromosome passenger complex as SUMOylation targets, which lead to dampening of error correction pathways. During mitosis, sister chromatids attach to microtubules from opposite poles, called biorientation. Sister chromatid cohesion resists microtubule forces, generating tension, which provides the signal that biorientation has occurred. How tension silences the surveillance pathways that prevent cell cycle progression and correct erroneous kinetochore–microtubule attachments remains unclear. Here we show that SUMOylation dampens error correction to allow stable sister kinetochore biorientation and timely anaphase onset. The Siz1/Siz2 SUMO ligases modify the pericentromere-localized shugoshin (Sgo1) protein before its tension-dependent release from chromatin. Sgo1 SUMOylation reduces its binding to protein phosphatase 2A (PP2A), and weakening of this interaction is important for stable biorientation. Unstable biorientation in SUMO-deficient cells is associated with persistence of the chromosome passenger complex (CPC) at centromeres, and SUMOylation of CPC subunit Bir1 also contributes to timely anaphase onset. We propose that SUMOylation acts in a combinatorial manner to facilitate dismantling of the error correction machinery within pericentromeres and thereby sharpen the metaphase–anaphase transition. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1083/jcb.202005130 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f23b8884a48798f234c67bf306c481d https://hdl.handle.net/21.11116/0000-0008-A39F-D |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9f23b8884a48798f234c67bf306c481d |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1083/jcb.202005130 |
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