Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA
| العنوان: | Cytotoxicity of intracanal dressings on apical papilla cells differ upon activation with E. faecalis LTA |
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| المؤلفون: | Celso Luiz Caldeira, Carla Renata Sipert, Aline Pereira de Oliveira |
| المصدر: | Journal of Applied Oral Science, Volume: 27, Article number: e20180291, Published: 21 FEB 2019 Journal of Applied Oral Science v.27 2019 Journal of applied oral science Universidade de São Paulo (USP) instacron:USP Journal of Applied Oral Science Journal of Applied Oral Science, Vol 27, Iss 0 (2019) |
| بيانات النشر: | Faculdade De Odontologia De Bauru - USP, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Lipopolysaccharides, Male, Time Factors, Adolescent, Cell Survival, Context (language use), Enterococcus faecalis, Calcium Hydroxide, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Tooth Apex, Ciprofloxacin, Metronidazole, Cytotoxic T cell, Humans, MTT assay, Viability assay, Cefaclor, Cytotoxicity, General Dentistry, Dental Papilla, Cells, Cultured, Analysis of Variance, Immunity, Cellular, biology, Root Canal Irrigants, Chemistry, Reproducibility of Results, 030206 dentistry, biology.organism_classification, Dental pulp cavity, Molecular biology, Anti-Bacterial Agents, lcsh:RK1-715, Teichoic Acids, Cell culture, lcsh:Dentistry, Female, Original Article, Lipoteichoic acid, Cell culture techniques |
| الوصف: | Objective The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC). Material and Methods Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP – Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC – 1:1:2) and modified CMC (mCMC – 2:2:1) by using MTT assay. The substances were reconstituted in DMEM at 1,000 µg/mL and ¼ serially diluted before being kept in contact with cells for 1, 3, 5 and 7 days. Further, cells were primed with 1 µg/mL of Enterococcus faecalis LTA for 7 days prior to the viability test with 1,000 µg/mL of each substance. Statistical analysis was performed using one-way analysis of variance (ANOVA) and two-way ANOVA respectively followed by Tukey’s post-test. Significance levels were set at p |
| وصف الملف: | text/html |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f08f4b05d19ee48d4f391cef6f76e6d http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572019000100433&lng=en&tlng=en |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9f08f4b05d19ee48d4f391cef6f76e6d |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |