Characterization of otoconin-95, the major protein of murine otoconia, provides insights into the formation of these inner ear biominerals
| العنوان: | Characterization of otoconin-95, the major protein of murine otoconia, provides insights into the formation of these inner ear biominerals |
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| المؤلفون: | Michel Leibovici, Elisabeth Verpy, Christine Petit |
| المصدر: | Proceedings of the National Academy of Sciences. 96:529-534 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Molecular Sequence Data, Fluorescent Antibody Technique, Otolithic membrane, Biology, Phospholipases A, Embryonic and Fetal Development, Mice, Otolithic Membrane, Calcification, Physiologic, Complementary DNA, otorhinolaryngologic diseases, medicine, Animals, Inner ear, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, In Situ Hybridization, Cochlea, Glycoproteins, chemistry.chemical_classification, Extracellular Matrix Proteins, Multidisciplinary, Base Sequence, Calcium-Binding Proteins, Alternative splicing, Gene Expression Regulation, Developmental, Ear, Sequence Analysis, DNA, Biological Sciences, Immunohistochemistry, Molecular biology, Embryonic stem cell, Cell biology, Alternative Splicing, medicine.anatomical_structure, chemistry, RNA splicing, sense organs, Glycoprotein |
| الوصف: | During the course of a study aimed at identifying inner ear-specific transcripts, a 1,906-bp murine cDNA predicted to encode a secreted 469-aa protein with two domains of homology with the secreted phospholipases A 2 was isolated. This transcript is specifically expressed in the inner ear from embryonic day 9.5. The encoded 95-kDa glycoprotein is the major protein of the utricular and saccular otoconia and thus was named otoconin-95. By immunohistofluorescence, otoconin-95 also was detected in the cupulae of the semicircular canals and in previously undescribed transient granular structures of the cochlea. Otoconin-95 was found to be synthesized by various nonsensory cell types, but not by the supporting cells of the sensory epithelia, which produce the otoconial precursor vesicles. In addition, multiple isoforms generated by differential splicing were observed in different combinations during development. Based on the present results, we propose a model for the formation of the otoconia. |
| تدمد: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.96.2.529 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ebf162af262b1a18774aade3697caa8 https://doi.org/10.1073/pnas.96.2.529 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9ebf162af262b1a18774aade3697caa8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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| DOI: | 10.1073/pnas.96.2.529 |