Clonality of Primary Pulmonary Lymphoproliferative Disorders; Using in Situ Hybridization and Polymerase Chain Reaction for Immunoglobulin
| العنوان: | Clonality of Primary Pulmonary Lymphoproliferative Disorders; Using in Situ Hybridization and Polymerase Chain Reaction for Immunoglobulin |
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| المؤلفون: | Masahiro Kikuchi, Midori Sugihara, Koichi Ohshima, Takayuki Shirakusa, M Yahiro, Kotaro Tashiro, Junji Suzumiya, Satoshi Yoneda |
| المصدر: | Leukemia & Lymphoma. 36:157-167 |
| بيانات النشر: | Informa UK Limited, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Adult, Lung Diseases, Male, Cancer Research, Pathology, medicine.medical_specialty, Population, Lymphoproliferative disorders, In situ hybridization, Biology, Immunoglobulin light chain, Polymerase Chain Reaction, law.invention, law, medicine, Humans, education, In Situ Hybridization, Polymerase chain reaction, B cell, Aged, Aged, 80 and over, education.field_of_study, Genes, Immunoglobulin, Hematology, Middle Aged, medicine.disease, Immunohistochemistry, Molecular biology, Lymphoproliferative Disorders, Blotting, Southern, medicine.anatomical_structure, Oncology, biology.protein, Immunoglobulin heavy chain, Female, Immunoglobulin Light Chains, Antibody, Immunoglobulin Heavy Chains |
| الوصف: | Primary pulmonary lymphoproliferative disorders (PLDs) are histologically divided into a neoplastic state of high and low grade malignant lymphoma (ML), and a reactive state of follicular bronchitis/bronchiolitis (FB) and lymphoid interstitial pneumonia (LIP). We reviewed 19 cases with PLDs, including 4 cases each of high and low grade B cell ML, 6 FB cases, and 5 cases of LIP. To clarify the clonality of the proliferating cells, we performed an immunohistochemical examination (IHC), in situ hybridization (ISH) for the immunoglobulin light chain and a polymerase chain reaction (PCR) analysis of the immunoglobulin heavy chain gene using DNA obtained from paraffin sections. In addition, a Southern blot analysis was also performed in 6 cases using fresh materials. In IHC, all ML were positive for L26 (CD20), while the monoclonality of the kappa light chain was observed in only one high grade case. However, using ISH we could detect the clonality in three of four high grade ML cases and in one of four low grade ML cases. In FB and LIP, no clonality of immunoglobulin by ISH was observed. In a PCR analysis for the immunoglobulin heavy chain gene, we could detect one or two prominent bands in all 8 cases of high and low grade ML. On the other hand, in all cases of FB and LIP, we could only detect either an oligoclonal or polyclonal population. In summary, the presence of monoclonality of ISH and/or PCR for the immunoglobulin heavy chain gene were limited in the neoplastic state, but not in the reactive state. |
| تدمد: | 1029-2403 1042-8194 |
| DOI: | 10.3109/10428199909145960 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ebb95aa0bbdd655d6a533cf2e808b49 https://doi.org/10.3109/10428199909145960 |
| رقم الانضمام: | edsair.doi.dedup.....9ebb95aa0bbdd655d6a533cf2e808b49 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10292403 10428194 |
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| DOI: | 10.3109/10428199909145960 |