The Photosystem II Assembly Factor Ycf48 from the Cyanobacterium Synechocystis sp. PCC 6803 Is Lipidated Using an Atypical Lipobox Sequence
| العنوان: | The Photosystem II Assembly Factor Ycf48 from the Cyanobacterium Synechocystis sp. PCC 6803 Is Lipidated Using an Atypical Lipobox Sequence |
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| المؤلفون: | Jianfeng Yu, Jana Knoppová, Josef Komenda, Peter J. Nixon, Petr Halada, Jan Janouškovec, Julian P. Whitelegge |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 3733, p 3733 (2021) International journal of molecular sciences, vol 22, iss 7 International Journal of Molecular Sciences Volume 22 Issue 7 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Cyanobacteria, chlorophyll-binding proteins, Photosystem II, 1.1 Normal biological development and functioning, macromolecular substances, Photosynthesis, 01 natural sciences, Article, Catalysis, Inorganic Chemistry, lcsh:Chemistry, 03 medical and health sciences, Residue (chemistry), Bacterial Proteins, Underpinning research, Genetics, Physical and Theoretical Chemistry, Molecular Biology, lcsh:QH301-705.5, Spectroscopy, chemistry.chemical_classification, Chemical Physics, photosynthesis, biology, Organic Chemistry, Mutagenesis, Synechocystis, Photosystem II Protein Complex, photosystem II, General Medicine, biology.organism_classification, Lipid Metabolism, Computer Science Applications, Amino acid, 030104 developmental biology, chemistry, Biochemistry, lcsh:Biology (General), lcsh:QD1-999, Generic health relevance, Other Biological Sciences, Other Chemical Sciences, Biogenesis, 010606 plant biology & botany, Cysteine |
| الوصف: | Photochemical energy conversion during oxygenic photosynthesis is performed by membrane-embedded chlorophyll-binding protein complexes. The biogenesis and maintenance of these complexes requires auxiliary protein factors that optimize the assembly process and protect nascent complexes from photodamage. In cyanobacteria, several lipoproteins contribute to the biogenesis and function of the photosystem II (PSII) complex. They include CyanoP, CyanoQ, and Psb27, which are all attached to the lumenal side of PSII complexes. Here, we show that the lumenal Ycf48 assembly factor found in the cyanobacterium Synechocystis sp. PCC 6803 is also a lipoprotein. Detailed mass spectrometric analysis of the isolated protein supported by site-directed mutagenesis experiments indicates lipidation of the N-terminal C29 residue of Ycf48 and removal of three amino acids from the C-terminus. The lipobox sequence in Ycf48 contains a cysteine residue at the −3 position compared to Leu/Val/Ile residues found in the canonical lipobox sequence. The atypical Ycf48 lipobox sequence is present in most cyanobacteria but is absent in eukaryotes. A possible role for lipoproteins in the coordinated assembly of cyanobacterial PSII is discussed. |
| وصف الملف: | application/pdf; text |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e9aaad6b6bc3d9ffb4cdce4b412c11b https://www.mdpi.com/1422-0067/22/7/3733 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9e9aaad6b6bc3d9ffb4cdce4b412c11b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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