Isolation of Human Renin Inhibitor from Soybean: Soyasaponin I Is the Novel Human Renin Inhibitor in Soybean
| العنوان: | Isolation of Human Renin Inhibitor from Soybean: Soyasaponin I Is the Novel Human Renin Inhibitor in Soybean |
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| المؤلفون: | Kazuyuki Hori, Seihan Yamada, Saori Takahashi, Mamoru Shinbo, Kazuyuki Hiwatashi, Takeshi Gotoh |
| المصدر: | Bioscience, Biotechnology, and Biochemistry. 72:3232-3236 |
| بيانات النشر: | Informa UK Limited, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Male, medicine.medical_specialty, medicine.drug_class, Blood Pressure, Biology, Applied Microbiology and Biotechnology, Biochemistry, Renin inhibitor, Analytical Chemistry, Inhibitory Concentration 50, Non-competitive inhibition, Internal medicine, Renin, parasitic diseases, Renin–angiotensin system, medicine, Animals, Humans, Amino Acid Sequence, Enzyme Inhibitors, Oleanolic Acid, Molecular Biology, IC50, Organic Chemistry, Angiotensin-converting enzyme, social sciences, General Medicine, Kallikrein, Saponins, Trypsin, Rats, Kinetics, Endocrinology, Hypertension, Cardiovascular agent, biology.protein, Soybeans, geographic locations, Biotechnology, medicine.drug |
| الوصف: | We found human renin inhibitory activity in soybean and isolated the active compound, soybean renin inhibitor (SRI). The physico-chemical data on the isolated SRI were identical with those of soyasaponin I. SRI showed significant inhibition against recombinant human renin, with an IC(50) value of 30 microg/ml. Kinetic studies with SRI indicated partial noncompetitive inhibition, with a K(i) value of 37.5 microM. On the other hand, SRI weakly inhibited pepsin, papain, and bromeline activities, but did not inhibit other proteinases, such as trypsin, kallikrein, angiotensin converting enzyme, and aminopeptidase M. Moreover, a significant (p0.05) decrease in the systolic blood pressure of spontaneously hypertensive rats was observed when partially purified SRI was orally administrated at 40 mg/kg/d for 7 weeks. This is the first demonstration of a renin inhibitor from soybean, soyasaponin I. |
| تدمد: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.80495 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9df8902f63fa70b9b3e0091d0efdeaa9 https://doi.org/10.1271/bbb.80495 |
| رقم الانضمام: | edsair.doi.dedup.....9df8902f63fa70b9b3e0091d0efdeaa9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13476947 09168451 |
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| DOI: | 10.1271/bbb.80495 |