Enzymatic Time-Temperature Indicator Prototype Developed by Immobilizing Laccase on Electrospun Fibers to Predict Lactic Acid Bacterial Growth in Milk during Storage
| العنوان: | Enzymatic Time-Temperature Indicator Prototype Developed by Immobilizing Laccase on Electrospun Fibers to Predict Lactic Acid Bacterial Growth in Milk during Storage |
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| المؤلفون: | Shih-Hsin Chen, Shyi-Neng Lou, Hui-Huang Chen, Li-Chen Chen, Shih-Bin Lin, Ting-Yu Tsai, Yen-Hui Chen |
| المصدر: | Nanomaterials Volume 11 Issue 5 Nanomaterials, Vol 11, Iss 1160, p 1160 (2021) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, TTI, General Chemical Engineering, Bacterial growth, 01 natural sciences, Polyvinyl alcohol, Article, laccase, Chitosan, chemistry.chemical_compound, 0404 agricultural biotechnology, 010608 biotechnology, General Materials Science, QD1-999, electrospinning, Laccase, milk, Time temperature indicator, Chromatography, 04 agricultural and veterinary sciences, 040401 food science, Electrospinning, Lactic acid, lactic acid bacteria, Chemistry, chemistry, Guaiacol |
| الوصف: | Laccase was immobilized on a chitosan/polyvinyl alcohol/tetraethylorthosilicate electrospun film (ceCPTL) and colored with guaiacol to obtain a laccase time–temperature indicator (TTI) prototype. The activation energy (Ea) of coloration of the prototype was 50.89–33.62 kJ/mol when 8–25 μg/cm2 laccase was immobilized on ceCPTL, and that of lactic acid bacteria (LAB) growth in milk was 73.32 kJ/mol. The Ea of coloration of the TTI prototype onto which 8–10 μg/cm2 laccase was immobilized was in the required range for predicting LAB growth in milk. The coloration endpoint of the TTI prototype onto which 10 μg/cm2 (0.01 U) laccase was immobilized could respond to the LAB count reaching 106 colony-forming units (CFU)/mL in milk during a static temperature response test, and the prediction error was discovered to be low. In dynamic temperature response experiments with intermittent temperature changes between 4 and 25 °C, the coloration rate of the laccase TTI prototype was consistent with LAB growth. The results of this study indicate that the laccase TTI prototype can be applied as a visual monitoring indicator to assist in evaluating milk quality in cold chains. |
| وصف الملف: | application/pdf |
| تدمد: | 2079-4991 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9da92b2bc773ac8f066e43b63831616e https://pubmed.ncbi.nlm.nih.gov/33946708 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9da92b2bc773ac8f066e43b63831616e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20794991 |
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