Direct E-DNA sensor of Mycobacterium tuberculosis mutant strain based on new nanocomposite transducer (Fc-ac-OMPA/MWCNTs)
| العنوان: | Direct E-DNA sensor of Mycobacterium tuberculosis mutant strain based on new nanocomposite transducer (Fc-ac-OMPA/MWCNTs) |
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| المؤلفون: | Ayoub Haj Said, Saber Blili, Hafsa Korri-Youssoufi, S. Bizid, Rym Mlika |
| المساهمون: | Laboratoire des Interfaces et Matériaux Avancés [Monastir] (LIMA), Faculté des Sciences de Monastir (FSM), Université de Monastir - University of Monastir (UM)-Université de Monastir - University of Monastir (UM), Laboratoire Polymères, Biopolymères, Matériaux Organiques [Monastir] (LPBMO), Université de Monastir - University of Monastir (UM), Equipe de Chimie Bioorganique et Bioinorganique, Institut de Chimie Moléculaire et des Matériaux d'Orsay (ICMMO), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC) |
| المصدر: | Talanta Talanta, Elsevier, 2018, 184, pp.475-483. ⟨10.1016/j.talanta.2018.03.025⟩ |
| بيانات النشر: | Elsevier BV, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | DNA, Bacterial, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Biosensing Techniques, Microbial Sensitivity Tests, 02 engineering and technology, Polymorphism, Single Nucleotide, 01 natural sciences, Oligomer, Nanocomposites, Analytical Chemistry, Redox indicator, Mycobacterium tuberculosis, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Drug Resistance, Bacterial, ComputingMilieux_MISCELLANEOUS, Nanocomposite, biology, DNA–DNA hybridization, Hybridization probe, 010401 analytical chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, biology.organism_classification, 0104 chemical sciences, 3. Good health, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, chemistry, Mutation, Biophysics, Rifampin, 0210 nano-technology, Biosensor, DNA |
| الوصف: | Direct DNA sensor based on new nanocomposite materials (Fc-ac-OMPA/MWCNTs) has been investigated. This nanocomposite was formed by combining the redox oligomer “oligo-methoxy-phenyl-acetonitrile” (Fc-ac-OMPA) and the MWCNTs via a simple π-π stacking interaction in the aim to ameliorate the biosensor performance. The redox indicator and the functional groups of the redox oligomer have been used for monitoring the electrochemical behavior and the flexibility for direct covalent attachment of Hepatitis C DNA probe. This nanocomposite shows high performance of DNA hybridization with a detection limit of 0.08 fmol L−1. Moreover, the biosensor was applied for the detection of pathogenic bacterium such as DNA from Mycobacterium tuberculosis strand. Developed biosensor has been able to detect a single nucleotide polymorphism (SNP)T (TCG/ TTG) which confers resistance of M. tuberculosis to rifampicin drug. |
| تدمد: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2018.03.025 |
| DOI: | 10.1016/j.talanta.2018.03.025⟩ |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d7a448c00c5ccc3f08b6bfbbab1f26e https://doi.org/10.1016/j.talanta.2018.03.025 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....9d7a448c00c5ccc3f08b6bfbbab1f26e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00399140 |
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| DOI: | 10.1016/j.talanta.2018.03.025 |