Fertility of Pedicellate Spikelets in Sorghum Is Controlled by a Jasmonic Acid Regulatory Module
| العنوان: | Fertility of Pedicellate Spikelets in Sorghum Is Controlled by a Jasmonic Acid Regulatory Module |
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| المؤلفون: | Chad Hayes, Michael Regulski, Doreen Ware, John J. Burke, Nicholas Gladman, Zhanguo Xin, Junping Chen, Lifang Zhang, Young Koung Lee, Gloria Burow, Shawn A. Christensen, Yinping Jiao, Lavanya Dampanaboina, Ratan Chopra |
| المصدر: | International Journal of Molecular Sciences Volume 20 Issue 19 International Journal of Molecular Sciences, Vol 20, Iss 19, p 4951 (2019) |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Mutant, 01 natural sciences, lcsh:Chemistry, chemistry.chemical_compound, Gene Expression Regulation, Plant, Promoter Regions, Genetic, lcsh:QH301-705.5, Spectroscopy, Phylogeny, jasmonic acid signaling, Plant Proteins, biology, Jasmonic acid, food and beverages, Methane sulfonate, General Medicine, Computer Science Applications, Cell biology, Horticulture, Inflorescence, Metabolic Networks and Pathways, Protein Binding, Plant Development, Cyclopentanes, Catalysis, Article, Inorganic Chemistry, 03 medical and health sciences, Amino Acid Sequence, Oxylipins, Physical and Theoretical Chemistry, Molecular Biology, Transcription factor, Sorghum, Panicle, Binding Sites, Crop yield, Gene Expression Profiling, Organic Chemistry, Promoter, Meristem, biology.organism_classification, transcriptional regulators, 030104 developmental biology, Fertility, lcsh:Biology (General), lcsh:QD1-999, chemistry, gene expression, Edible Grain, Sweet sorghum, 010606 plant biology & botany, Transcription Factors |
| الوصف: | As in other cereal crops, the panicles of sorghum (Sorghum bicolor (L.) Moench) comprise two types of floral spikelets (grass flowers). Only sessile spikelets (SSs) are capable of producing viable grains, whereas pedicellate spikelets (PSs) cease development after initiation and eventually abort. Consequently, grain number per panicle (GNP) is lower than the total number of flowers produced per panicle. The mechanism underlying this differential fertility is not well understood. To investigate this issue, we isolated a series of EMS-induced multiseeded (msd) mutants that result in full spikelet fertility, effectively doubling GNP. Previously, we showed that MSD1 is a TCP (Teosinte branched/Cycloidea/PCF) transcription factor that regulates jasmonic acid (JA) biosynthesis, and ultimately floral sex organ development. Here, we show that MSD2 encodes a lipoxygenase (LOX) that catalyzes the first committed step of JA biosynthesis. Further, we demonstrate that MSD1 binds to the promoters of MSD2 and other JA pathway genes. Together, these results show that a JA-induced module regulates sorghum panicle development and spikelet fertility. The findings advance our understanding of inflorescence development and could lead to new strategies for increasing GNP and grain yield in sorghum and other cereal crops.SignificanceThrough a single base pair mutation, grain number can be increased by ~200% in the globally important crop Sorghum bicolor. This mutation affects the expression of an enzyme, MSD2, that catalyzes the jasmonic acid pathway in developing floral meristems. The global gene expression profile in this enzymatic mutant is similar to that of a transcription factor mutant, msd1, indicating that disturbing any component of this regulatory module disrupts a positive feedback loop that occurs normally due to regular developmental perception of jasmonic acid. Additionally, the MSD1 transcription factor is able to regulate MSD2 in addition to other jasmonic acid pathway genes, suggesting that it is a primary transcriptional regulator of this hormone signaling pathway in floral meristems. |
| وصف الملف: | application/pdf |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d6909482f46e99fc2ef3b0c496de1f3 https://pubmed.ncbi.nlm.nih.gov/31597271 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9d6909482f46e99fc2ef3b0c496de1f3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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